Platelet-Derived Growth Factor Isoforms PDGF-AA, PDGF-AB and PDGF-BB Exert Specific Effects on Collagen Gene Expression and Mitotic Activity of Cultured Human Wound Fibroblasts
| Autor: | J. Lepisto, J. Niinikoski, Marko Vähä-Kreula, M. Laato, J. Peltonen |
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| Rok vydání: | 1995 |
| Předmět: |
Gene isoform
Platelet-derived growth factor Becaplermin Biophysics Gene Expression Alpha (ethology) In Vitro Techniques Biochemistry Structure-Activity Relationship chemistry.chemical_compound Gene expression Humans RNA Messenger Molecular Biology Cells Cultured Platelet-Derived Growth Factor Wound Healing Dose-Response Relationship Drug biology Cell growth Proto-Oncogene Proteins c-sis Cell Biology Fibroblasts Molecular biology Procollagen peptidase chemistry biology.protein Collagen Wound healing Cell Division Platelet-derived growth factor receptor |
| Zdroj: | Biochemical and Biophysical Research Communications. 209:393-399 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.1516 |
| Popis: | This study was designed to clarify the action of three different isoforms of PDGF (PDGF-AA, PDGF-AB and PDGF-BB) on the proliferation rate and collagen synthesis of fibroblasts cultured from normal human wounds. PDGF-AA and PDGF-BB down-regulated both the steady-state levels of pro alpha 1 (I) and pro alpha 1 (III) collagen chain mRNAs and the production of collagen, both in a dose-dependent manner. Interestingly, low concentrations (1 ng/ml) of PDGF-AB up-regulated the expression of type I and III procollagen mRNAs by cultured wound fibroblasts, while under high concentrations (30 ng/ml) of PDGF-AB this effect changed to the opposite. The proliferation rate of wound fibroblasts was stimulated by PDGF-BB which elicited a dose-dependent (1-30 ng/ml) stimulation of cell proliferation, whereas PDGF-AB and -AA were less effective in this respect. |
| Databáze: | OpenAIRE |
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