Role of Shiga Toxins in Cytotoxicity and Immunomodulatory Effects of Escherichia coli O157:H7 during Host-Bacterial Interactions in vitro
| Autor: | Romina Jimena Fernandez-Brando, Carolina Maiumi Shiromizu, Gonzalo Ezequiel Pineda, Analía Silvina Trevani, Marina S. Palermo, Andrea Cecilia Bruballa, Manuel J. Muñoz, Leticia V. Bentancor, Florencia Sabbione, María Victoria Ramos, Alan Mauro Bernal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
il-1β
Health Toxicology and Mutagenesis lcsh:Medicine Inflammation ehec Toxicology medicine.disease_cause Microbiology Proinflammatory cytokine 03 medical and health sciences IL-1Β il-8 hemic and lymphatic diseases MRNA medicine Interleukin 8 MACROPHAGES Escherichia coli Cell damage 030304 developmental biology 0303 health sciences IL-8 biology mrna 030306 microbiology lcsh:R Shiga toxin purl.org/becyt/ford/3.1 [https] biochemical phenomena metabolism and nutrition stx2 bacterial infections and mycoses biology.organism_classification medicine.disease macrophages biology.protein EHEC bacteria purl.org/becyt/ford/3 [https] intestinal epithelial cells Cytokine secretion INTESTINAL EPITHELIAL CELLS medicine.symptom STX2 Bacteria |
| Zdroj: | Toxins, Vol 12, Iss 1, p 48 (2020) Toxins Volume 12 Issue 1 CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins12010048 |
| Popis: | Enterohemorrhagic Escherichia coli (EHEC) strains are food-borne pathogens that can cause different clinical conditions. Shiga toxin 2a and/or 2c (Stx2)-producing E. coli O157:H7 is the serotype most frequently associated with severe human disease. In this work we analyzed the hypothesis that host cells participate in Stx2 production, cell damage, and inflammation during EHEC infection. With this aim, macrophage-differentiated THP-1 cells and the intestinal epithelial cell line HCT-8 were incubated with E. coli O157:H7. A time course analysis of cellular and bacterial survival, Stx2 production, stx2 transcription, and cytokine secretion were analyzed in both human cell lines. We demonstrated that macrophages are able to internalize and kill EHEC. Simultaneously, Stx2 produced by internalized bacteria played a major role in macrophage death. In contrast, HCT-8 cells were completely resistant to EHEC infection. Besides, macrophages and HCT-8 infected cells produce IL-1&beta and IL-8 inflammatory cytokines, respectively. At the same time, bacterial stx2-specific transcripts were detected only in macrophages after EHEC infection. The interplay between bacteria and host cells led to Stx production, triggering of inflammatory response and cell damage, all of which could contribute to a severe outcome after EHEC infections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |