Identification of Polymorphisms in the Rabbit Growth Hormone Receptor (GHR) Gene and Association with Finishing Weight in a Commercial Meat Rabbit Line
| Autor: | Emilio Scotti, Andrea Frabetti, Giuseppe Sparacino, Valerio Joe Utzeri, Stefania Dall'Olio, Daniela Fornasini, Luca Fontanesi |
|---|---|
| Přispěvatelé: | Fontanesi, L., Sparacino, G., Utzeri, V.J., Scotti, E., Fornasini, D., Dall’Olio, S., Frabetti, A |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
candidate gene approach Male Candidate gene Meat Bioengineering Single-nucleotide polymorphism Growth hormone receptor Biology Oryctolagus cuniculus Polymorphism Single Nucleotide 03 medical and health sciences Exon meat production Genotype Animals Amino Acid Sequence Allele frequency Gene Genetic Association Studies Genetics missense mutation Body Weight 0402 animal and dairy science 04 agricultural and veterinary sciences Receptors Somatotropin 040201 dairy & animal science Association study 030104 developmental biology Genetic marker Animal Science and Zoology Female Rabbits Sequence Alignment Biotechnology |
| Zdroj: | Animal biotechnology. 27(2) |
| ISSN: | 1532-2378 |
| Popis: | A shortcut to identify DNA markers associated with economic traits is to use a candidate gene approach that is still useful in livestock species in which molecular tools and resources are not advanced or not well developed. Mutations in the growth hormone receptor (GHR) gene associated with production traits have been already described in several livestock species. For this reason GHR could be an interesting candidate gene in the rabbit. In this study we re-sequenced all exons and non-coding regions of the rabbit GHR gene in a panel of 10 different rabbits and identified 10 single nucleotide polymorphisms (SNPs). One of them (g.63453192C>G or c.106C>G), located in exon 3 was a missense mutation (p.L36V) substituting an amino acid in a highly conserved position across all mammals. This mutation was genotyped in 297 performance tested rabbits of a meat male line and association analysis showed that the investigated SNP was associated with weight at 70 days (P < 0.05). The most frequent genotype (GG) was in animals with higher weight at this age, suggesting that the high directional selection pressure toward this trait since the constitution of the genotyped line might have contributed to shape allele frequencies at this polymorphic site. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|