Clinical evaluation of a new in-vitro assay for specific IgE, the immuno CAP system
| Autor: | M. Seppey, B. Mosimann, Vincent Aubert, Y. Jaccard, Alain Pécoud, R. Peitrequin, M. Claeys, Marie‐Pierre Nisoli, Annette Leimgruber |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Allergy Adolescent Immunology Immunoelectrophoresis Immunoglobulin E Radioallergosorbent Test immune system diseases Immunopathology otorhinolaryngologic diseases medicine Humans Immunology and Allergy Aged Skin Tests Asthma medicine.diagnostic_test biology business.industry Radioallergosorbent test Middle Aged medicine.disease In vitro respiratory tract diseases Immunologic Techniques biology.protein Female Phadebas business Immunoelectrophoresis Two-Dimensional |
| Zdroj: | Clinical Experimental Allergy. 21:127-131 |
| ISSN: | 1365-2222 0954-7894 |
| DOI: | 10.1111/j.1365-2222.1991.tb00814.x |
| Popis: | The Pharmacia CAP system is a new assay for specific IgE characterized by a new solid phase (hydrophilic polymer encased in a capsule). The CAP results were compared to those of the Phadebas IgE RAST and skin-prick tests (SPT) performed in 145 subjects suffering from rhinitis and/or asthma with eight common inhalant allergens (total number of tests = 1160). Concording CAP/RAST results were found in 91% of the tests. The CAP was positive in 78% and the RAST in 65% of the positive SPT. Conversely, the CAP was negative in 90.6% and the RAST in 96.4% of the negative SPT. A pattern negative SPT, negative RAST and positive CAP' was found in 56 tests (40 subjects): in four such subjects, the CAP positivity was confirmed by a positive IgE crossed radioimmunoelectrophoresis. Three borderline positive results were found among 240 negative controls (serum from cord blood or non-atopics). These data indicate that compared with SPT the CAP system is (a) more sensitive than the Pharmacia RAST and (b) does not seem less specific. |
| Databáze: | OpenAIRE |
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