Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere
| Autor: | Diego Bonaldo Genuário, Harold Alexander Vargas Hoyos, Itamar Soares de Melo, Suikinai Nobre Santos, Fábio Sérgio Paulino da Silva, Tiago Domingues Zucchi, Leonardo José da Silva |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Streptomyces cavourensis DNA Bacterial Sequence analysis Biology 010603 evolutionary biology 01 natural sciences Microbiology Streptomyces 03 medical and health sciences RNA Ribosomal 16S Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology Triticum Genetics Base Composition Phylogenetic tree Strain (biology) Fatty Acids General Medicine Sequence Analysis DNA rpoB biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Genes Bacterial Rhizosphere Streptomyces araujoniae Brazil Multilocus Sequence Typing |
| Zdroj: | International journal of systematic and evolutionary microbiology. 69(8) |
| ISSN: | 1466-5034 |
| Popis: | A novel actinobacterium, designated strain 1AS2cT, was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026T, Streptomyces albolongus NBRC 13465T and Streptomyces araujoniae ASBV-1T, sharing a similarity value of 98.2, 98.1 and 97.9 %, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA–DNA relatedness between strain 1AS2cT and its closest phylogenetic neighbours ( S. cavourensis NBRC 13026T, S. albolongus NBRC 13465T and S. araujoniae ASBV-1T: 56, 62.5 and 63.0 %, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2cT to the genus Streptomyces , representing a novel species. It is concluded that strain 1AS2cT (=CMAA 1679T=NRRL B-65479T=DSM 105299T) can be classified as the type strain of a novel species of the genus Streptomyces , for which the name Streptomyces rhizosphaericola sp. nov. is proposed. |
| Databáze: | OpenAIRE |
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