The exocyst is required for melanin exocytosis from melanocytes and transfer to keratinocytes
| Autor: | Miguel C. Seabra, Liliana Bento-Lopes, Duarte C. Barral, Matilde V. Neto, Hugo Moreiras, Tiago C. Festas, Francisco J.C. Pereira |
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| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
0301 basic medicine Immunoprecipitation Phagocytosis Vesicular Transport Proteins Exocyst Dermatology Exocytosis General Biochemistry Genetics and Molecular Biology Cell Line Melanin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Humans Gene silencing Gene Silencing Melanosome Melanins integumentary system Cell biology Protein Subunits 030104 developmental biology Oncology chemistry 030220 oncology & carcinogenesis Melanocytes sense organs DNA Protein Binding |
| Zdroj: | Pigment Cell & Melanoma Research. 33:366-371 |
| ISSN: | 1755-148X 1755-1471 |
| Popis: | Skin pigmentation involves the production of the pigment melanin by melanocytes, in melanosomes and subsequent transfer to keratinocytes. Within keratinocytes, melanin polarizes to the apical perinuclear region to form a protective cap, shielding the DNA from ultraviolet radiation-induced damage. Previously, we found evidence to support the exocytosis by melanocytes of the melanin core, termed melanocore, followed by endo/phagocytosis by keratinocytes as a main form of transfer, with Rab11b playing a key role in the process. Here, we report the requirement for the exocyst tethering complex in melanocore exocytosis and transfer to keratinocytes. We observed that the silencing of the exocyst subunits Sec8 or Exo70 impairs melanocore exocytosis from melanocytes, without affecting melanin synthesis. Moreover, we confirmed by immunoprecipitation that Rab11b interacts with Sec8 in melanocytes. Furthermore, we found that the silencing of Sec8 or Exo70 in melanocytes impairs melanin transfer to keratinocytes. These results support our model as melanocore exocytosis from melanocytes is essential for melanin transfer to keratinocytes and skin pigmentation and suggest that the role of Rab11b in melanocore exocytosis is mediated by the exocyst. |
| Databáze: | OpenAIRE |
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