Cloning, expression analysis, and chromosomal mapping of GTPBP2, a novel member of the G protein family
| Autor: | Sumio Sugano, Hiroyuki Kato, Kenichi Yoshida, Munetomo Hida, Susumu Tateyama, Manabu Watanabe, Ryoji Yamaguchi, Kazuyuki Uchida |
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| Rok vydání: | 2000 |
| Předmět: |
Male
DNA Complementary G protein Molecular Sequence Data Gene Expression Biology Chromosomes Homology (biology) Mice Gene mapping GTP-Binding Proteins Testis Genetics Animals Humans Tissue Distribution Amino Acid Sequence RNA Messenger Cloning Molecular Peptide sequence Gene In Situ Hybridization Fluorescence Phylogeny Monomeric GTP-Binding Proteins Messenger RNA Sequence Homology Amino Acid Phylogenetic tree Chromosome Mapping Chromosome Sequence Analysis DNA General Medicine Blotting Northern Molecular biology Mice Inbred C57BL Chromosomes Human Pair 6 Female Sequence Alignment |
| Zdroj: | Gene. 256:51-58 |
| ISSN: | 0378-1119 |
| Popis: | We have identified a novel gene encoding a protein bearing GTP-binding motifs, the characteristics of GTP-binding proteins (G proteins). The deduced amino acid sequence exhibited the highest overall homology with GTPBP1 and its mouse orthologue GP-1. Hence, we named the gene GTPBP2. The mouse orthologue of this gene, Gtpbp2, showed 98% identity with GTPBP2 over the entire protein (the HGMW-approved nomenclature symbol is GTPBP2 and mouse orthologue is Gtpbp2). A phylogenetic analysis showed GTPBP2 and homologous G proteins (GTPBP1, AGP-1, and CGP-1) did not belong to major G protein families. They formed a distinct branch in the phylogenetic tree, suggesting that they constitute a novel G protein family. A 2.9 kb mRNA was predominantly detected in the testis along with various other organs. In situ hybridization analysis revealed that Gtpbp2 was predominantly expressed in spermatocytes and round-spermatids in the testis. These novel genes were localized to human chromosome 6p21.1–2 and mouse chromosome 17qC–D. |
| Databáze: | OpenAIRE |
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