Multiple free-radical scavenging (MULTIS) capacity in cattle serum
| Autor: | Taketoshi Asanuma, Go Kitahara, Anna Kimura, Shigeru Oowada, Yoshimi Sueishi, Erisa Kamogawa, Hiroyuki Satoh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Clinical Biochemistry Medicine (miscellaneous) Beef cattle 03 medical and health sciences chemistry.chemical_compound cattle serum multiple radical scavenging capacity Medicine Food science Scavenging Nutrition and Dietetics spin trapping Superoxide business.industry 0402 animal and dairy science Albumin Methyl radical 04 agricultural and veterinary sciences MULTIS 040201 dairy & animal science Biotechnology antioxidants 030104 developmental biology chemistry Uric acid Alkoxyl radicals Original Article Hydroxyl radical business |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| DOI: | 10.3164/jcbn.15-142 |
| Popis: | Multiple free-radical scavenging (MULTIS) activity in cattle and human sera was evaluated with electron spin resonance spectroscopy. Scavenging rates against six active species, namely hydroxyl radical, superoxide anion, alkoxyl radical, alkylperoxyl radical, methyl radical, and singlet oxygen were quantified. The difference in the electron spin resonance signal intensity in the presence and absence of the serum was converted into the scavenging rates. Comparative MULTIS measurements were made in sera from eight beef cattle, three fetal calves and fifteen healthy human volunteers. Further, we determined the MULTIS value of albumin, the most abundant component in serum. MULTIS values in cattle sera indicated higher scavenging activity against most free radical species tested than human sera. In particular, cattle serum scavenging activities against superoxide and methyl radical were higher than human serum by 2.6 and 3.7 fold, respectively. In cattle serum, albumin appears to play a dominant role in MULTIS activity, but in human serum that is not the case. Previous data indicated that the abundance of uric acid in bovine blood is nearly 80% less than humans; however, this difference does not explain the deviation in MULTIS profile. |
| Databáze: | OpenAIRE |
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