Prophylactic vaccination with phage-displayed epitope of C. albicans elicits protective immune responses against systemic candidiasis in C57BL/6 mice
| Autor: | Li Wang, Panyu Hua, Da-ning Lu, Qiong Yang, Jin-na Song, Rui-juan Gao, Da-wei Yuan |
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| Rok vydání: | 2005 |
| Předmět: |
CD4-Positive T-Lymphocytes
Phage display Epitope Microbiology Interferon-gamma Mice Inovirus Immune system Peptide Library Candida albicans Splenocyte medicine Animals Hypersensitivity Delayed HSP90 Heat-Shock Proteins Antibodies Fungal Heat-Shock Proteins General Veterinary General Immunology and Microbiology biology Candidiasis Public Health Environmental and Occupational Health biology.organism_classification medicine.disease Virology Corpus albicans Mice Inbred C57BL Infectious Diseases biology.protein Epitopes B-Lymphocyte Molecular Medicine Systemic candidiasis Antibody |
| Zdroj: | Vaccine. 23:4088-4096 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2004.07.005 |
| Popis: | Epitope LKVIRK on 47 kDa of heat shock protein (Hsp) 90 of Candida albicans, corresponding to residues 386-391 of the Hsp90, is recognized by patients recovering from invasive candidiasis. The efficacy of hybrid phage displaying epitope LKVIRK in the N-terminal region of the major coat protein (pVIII) in inducing anti-invasive candidiasis immune response was studied in C57BL/6 mice. Indirect phage-ELISA results demonstrated that the mice immunized with hybrid phage had significantly higher titers of epitope LKVIRK-specific serum IgG as compared to those immunized with heat-killed C. albicans (HK-CA). C57BL/6 mice immunized either with hybrid phage or with wild-type phage also developed significant levels of delayed-type hypersensitivity (DTH) response and splenocyte proliferation, as well as with HK-CA. In addition, high levels of IFN-gamma in the CD4(+) splenocytes from phage-immunized mice were detected as well during 1 week post-inoculation. Furthermore, mice immunized with hybrid phage acquired a resistance to systemic C. albicans infection as confirmed by fewer C. albicans cells in the kidneys, and had a longer lifespan compared to control groups following intravenous challenge with C. albicans. These results indicate that hybrid phage displaying epitope LKVIRK may serve as a potential vaccine conferring a resistance to systemic candidiasis. |
| Databáze: | OpenAIRE |
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