Volatiles from the fungal phytopathogen Penicillium aurantiogriseum modulate root metabolism and architecture through proteome resetting
| Autor: | Francisco Muñoz, Lukáš Spíchal, Nuria De Diego, Kinia Ameztoy, Irene García, Lydia Ugena, Goizeder Almagro, Edurne Baroja-Fernández, Abdellatif Bahaji, Samuel Gámez-Arcas, Luis C. Romero, Karel Doležal, Ángela María Sánchez-López, Javier Pozueta-Romero, Mohammad-Reza Hajirezaei, Pablo García-Gómez |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteomics Proteome Physiology Nitrogen assimilation Arabidopsis Plant Science Real-Time Polymerase Chain Reaction 01 natural sciences Plant Roots 03 medical and health sciences chemistry.chemical_compound Auxin Cell Wall Gene Expression Regulation Plant Hormone signaling Microbial volatile compounds Plant‐microbe interaction Cyanide scavenging Photosynthesis Penicillium aurantiogriseum Plant Diseases chemistry.chemical_classification Volatile Organic Compounds biology Arabidopsis Proteins Penicillium Metabolism biology.organism_classification Apoplast 030104 developmental biology Biostimulants chemistry Biochemistry Cytokinin Growth promotion 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Volatile compounds (VCs) emitted by the fungal phytopathogen Penicillium aurantiogriseum promote root growth and developmental changes in Arabidopsis. Here we characterized the metabolic and molecular responses of roots to fungal volatiles. Proteomic analyses revealed that these compounds reduce the levels of aquaporins, the iron carrier IRT1 and apoplastic peroxidases. Fungal VCs also increased the levels of enzymes involved in the production of mevalonate (MVA)‐derived isoprenoids, nitrogen assimilation and conversion of methionine to ethylene and cyanide. Consistently, fungal VC‐treated roots accumulated high levels of hydrogen peroxide (H2O2), MVA‐derived cytokinins, ethylene, cyanide and long‐distance nitrogen transport amino acids. qRT‐PCR analyses showed that many proteins differentially expressed by fungal VCs are encoded by VC non‐responsive genes. Expression patterns of hormone reporters and developmental characterization of mutants provided evidence for the involvement of cyanide scavenging and enhanced auxin, ethylene, cytokinin and H2O2 signaling in the root architecture changes promoted by fungal VCs. Our findings show that VCs from P. aurantiogriseum modify root metabolism and architecture, and improve nutrient and water use efficiencies through transcriptionally and non‐transcriptionally regulated proteome resetting mechanisms. Some of these mechanisms are subject to long‐distance regulation by photosynthesis and differ from those triggered by VCs emitted by beneficial microorganisms. |
| Databáze: | OpenAIRE |
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