Interleukin‐1β augments the angiogenesis of endothelial progenitor cells in an NF‐κB/CXCR7‐dependent manner
Autor: | Liangmiao Chen, Jiameng Huang, Da Sun, Xiaozhen Dai, Jun Chen, Xiangjuan Chen, Xiaoqing Yan, Shiyue Sun, Junhong He, Yi Tan, Lin Lin, Qiaoxia Dai, Luqing He, Xue Meng, Xia Fan, Chi Zhang |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Receptors CCR7 Stromal cell Angiogenesis medicine.medical_treatment Interleukin-1beta Models Biological angiogenesis interleukin‐1β 03 medical and health sciences chemistry.chemical_compound Chemokine receptor 0302 clinical medicine medicine Humans Progenitor cell Cells Cultured Endothelial Progenitor Cells Mitogen-Activated Protein Kinase 1 Tube formation Matrigel Mitogen-Activated Protein Kinase 3 Neovascularization Pathologic Chemistry NF-kappa B NF-κB Original Articles Cell Biology Cell biology extracellular signal‐regulated kinase‐1/2 030104 developmental biology Cytokine 030220 oncology & carcinogenesis CXC chemokine receptor 7 Molecular Medicine Original Article Biomarkers Signal Transduction |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | Endothelial progenitor cells (EPCs) are able to trigger angiogenesis, and pro‐inflammatory cytokines have beneficial effects on angiogenesis under physiological and pathological conditions. C‐X‐C chemokine receptor type 7 (CXCR‐7), receptor for stromal cell‐derived factor‐1, plays a critical role in enhancing EPC angiogenic function. Here, we examined whether CXCR7 mediates the pro‐angiogenic effects of the inflammatory cytokine interleukin‐1β (IL‐1β) in EPCs. EPCs were isolated by density gradient centrifugation and angiogenic capability was evaluated in vitro by Matrigel capillary formation assay and fibrin gel bead assay. IL‐1β elevated CXCR7 expression at both the transcriptional and translational levels in a dose‐ and time‐dependent manner, and blockade of the nuclear translocation of NF‐κB dramatically attenuated the IL‐1β‐mediated up‐regulation of CXCR7 expression. IL‐1β stimulation significantly promoted EPCs tube formation and this effect was largely impaired by CXCR7‐siRNA transfection. IL‐1β treatment stimulated extracellular signal‐regulated kinase 1/2 (Erk1/2) phosphorylation, and inhibition of Erk1/2 phosphorylation partially impaired IL‐1β‐induced tube formation of EPCs but without significant effects on CXCR7 expression. Moreover, blocking NF‐κB had no significant effects on IL‐1β‐stimulated Erk1/2 phosphorylation. These findings indicate that CXCR7 plays an important role in the IL‐1β‐enhanced angiogenic capability of EPCs and antagonizing CXCR7 is a potential strategy for inhibiting angiogenesis under inflammatory conditions. |
Databáze: | OpenAIRE |
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