Evaluation of different Mediterranean essential oils as prophylactic agents in anisakidosis

Autor: Gema Merino Espinosa, Concepción Navarro Moll, Adela Valero López, Magdalena Gómez-Mateos Pérez
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Mediterranean climate
Veterinary medicine
Cuminum
prophylactic action
Pharmaceutical Science
Anisakis
Artificial digestion
mediterranean plants
Origanum
Drug Discovery
Larva
biology
Mediterranean Region
Antinematodal Agents
Fishes
General Medicine
Lavandula
Parasitic disease
Molecular Medicine
Fish
animal structures
030106 microbiology
Anisakiasis
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
anisakis larvae
biocidal activity
Oils
Volatile
medicine
Animals
Plant Oils
Rats
Wistar
Pharmacology
Plants
Medicinal
Plant Extracts
business.industry
fungi
lcsh:RM1-950
biology.organism_classification
medicine.disease
Biotechnology
components of essential oils
Anisakidae
030104 developmental biology
lcsh:Therapeutics. Pharmacology
Complementary and alternative medicine
business
Phytotherapy
Olive oil
Zdroj: Pharmaceutical Biology, Vol 55, Iss 1, Pp 456-461 (2017)
ISSN: 1744-5116
1388-0209
Popis: Context: Anisakis Dujardin 1845 (Anisakidae) nematodes can cause gastrointestinal and allergic diseases when humans eat raw or undercooked seafood containing larvae. There is currently no drug available in the market against this parasitic disease, and the study of plant-derived molecules could be useful in the discovery of effective compounds. Objective: This research assesses the biocidal activity of a range of essential oils (EOs) from some Mediterranean plants against larvae found in the musculature of fresh fish. Materials and methods: EOs composition was analyzed by gas chromatography-mass spectroscopy. All the EOs were diluted at 5% v/v in olive oil to cover the fish with the solutions for 24 h. The larvae that abandoned the muscle and the larvae obtained from the artificial digestion of the fish were collected. Controls were carried out in parallel. Furthermore, Wistar rats were infected with the live larvae collected from the in vitro trials in order to find any larvae that may have penetrated the gastrointestinal wall. Results: Between 60.8% and 87.6% of parasites treated with EOs abandoned the fish muscle, and the highest in vitro mortality rate was achieved with oregano EO (53.9%). Rats previously treated with oregano, cumin and Spanish lavender EOs showed no detectable lesions in the digestive tract due to the infection with larvae. Conclusions: Oregano (Origanum vulgare L. Lamiaceae), cumin (Cuminum cyminum L. Apiaceae) and Spanish lavender (Lavender stoechas L. Lamiaceae) EOs could be used as promising ingredients in the development of products for the control of anisakiasis.
Databáze: OpenAIRE
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