Examination of surface polysaccharides ofActinobacillus pleuropneumoniaeserotype. 1 Grown under iron-restricted conditions
| Autor: | Mario Jacques, Daniel Dubreuil, Sonia-Élaine Paradis |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Serotype medicine.drug_class Iron Monoclonal antibody Polysaccharide Microbiology Flow cytometry Genetics medicine Serotyping Microscopy Immunoelectron Molecular Biology Actinobacillus pleuropneumoniae Bacterial Capsules chemistry.chemical_classification medicine.diagnostic_test Strain (chemistry) biology Polysaccharides Bacterial Antibodies Monoclonal O Antigens Flow Cytometry biology.organism_classification chemistry biology.protein Antibody Bacteria |
| Zdroj: | FEMS Microbiology Letters. 137:201-206 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1996.tb08106.x |
| Popis: | We investigated the expression of important Actinobacillus pleuropneumoniae surface polysaccharides, namely, capsular polysaccharides (CPS) and lipopolysaccharides (LPS), after growth under iron-restricted conditions. Iron restriction did not seem to affect the production of CPS, as determined by labelling with a monoclonal antibody (mAb) against the serotype 1 K-antigen and flow cytometry analysis, and also as determined by electron microscopy. SDS-PAGE revealed that the LPS profiles of these cells were also unaffected by iron restriction. Using flow cytometry analysis, however, we observed that binding of mAb against serotype 1 O-antigen was altered in cells of A. pleuropneumoniae serotype 1 reference strain (4074) grown under iron-restricted conditions. This strain exhibited two subpopulations with distinct patterns of reactivity with the mAb against the O-antigen. When strain 4074 was grown under iron-restricted conditions, a shift from one cell subpopulation (moderately fluorescent) to another cell subpopulation (highly fluorescent, thus binding more antibodies) was observed. Our results indicate that growth of A. pleuropneumoniae serotype 1 under iron-restricted conditions did not seem to affect CPS production, but might alter, at least for the reference strain, the expression of LPS. |
| Databáze: | OpenAIRE |
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