Pudendal but not tibial nerve stimulation inhibits bladder contractions induced by stimulation of pontine micturition center in cats
| Autor: | Richard C. Slater, Brian T. Kadow, Timothy D. Lyon, James R. Roppolo, Victor Chang, Zhaocun Zhang, Changfeng Tai, Bing Shen, Matthew C. Ferroni, William C. de Groat, Vladimir Lamm, Jicheng Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Detrusor muscle Male Physiology media_common.quotation_subject Pudendal nerve Adrenergic beta-Antagonists Urinary Bladder 030232 urology & nephrology Urination Stimulation 03 medical and health sciences Parasympathetic nervous system 0302 clinical medicine Parasympathetic Nervous System Physiology (medical) Neuromodulation Pons medicine Animals media_common Decerebrate State Urinary bladder Neural Control business.industry Muscle Smooth Propranolol Electric Stimulation Pudendal Nerve 030104 developmental biology medicine.anatomical_structure Anesthesia Bladder Disorder Cats Female Tibial Nerve business Spinal Nerve Roots Microelectrodes Muscle Contraction |
| Popis: | This study examined the possibility that pudendal nerve stimulation (PNS) or tibial nerve stimulation (TNS) inhibits the excitatory pathway from the pontine micturition center (PMC) to the urinary bladder. In decerebrate cats under α-chloralose anesthesia, electrical stimulation of the PMC (40 Hz frequency, 0.2-ms pulse width, 10–25 s duration) using a microelectrode induced bladder contractions >20 cmH2O amplitude when the bladder was filled to 60–70% capacity. PNS or TNS (5 Hz, 0.2 ms) at two and four times the threshold (2T and 4T) to induce anal or toe twitch was applied to inhibit the PMC stimulation-induced bladder contractions. Propranolol, a nonselective β-adrenergic receptor antagonist, was administered intravenously (1 mg/kg iv) to determine the role of sympathetic pathways in PNS/TNS inhibition. PNS at both 2T and 4T significantly ( P < 0.05) reduced the amplitude and area under the curve of the bladder contractions induced by PMC stimulation, while TNS at 4T facilitated the bladder contractions. Propranolol completely eliminated PNS inhibition and TNS facilitation. This study indicates that PNS, but not TNS, inhibits PMC stimulation-induced bladder contractions via a β-adrenergic mechanism that may occur in the detrusor muscle as a result of reflex activity in lumbar sympathetic nerves. Neither PNS nor TNS activated a central inhibitory pathway with synaptic connections to the sacral parasympathetic neurons that innervate the bladder. Understanding the site of action involved in bladder neuromodulation is important for developing new therapies for bladder disorders. |
| Databáze: | OpenAIRE |
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