A Serum Mannose-Binding Lectin Mediates Complement-Dependent Lysis of Influenza Virus-Infected Cells
| Autor: | E M Anders, Carol A. Hartley, R. A. B. Ezekowitz, Patrick C. Reading |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
viruses
Guinea Pigs Biophysics Hemagglutinin (influenza) Biology medicine.disease_cause Biochemistry Virus Antigenic drift Microbiology Classical complement pathway Orthomyxoviridae Infections Viral envelope Cricetinae Lectins Influenza A virus medicine Animals Complement Pathway Classical Molecular Biology Cells Cultured Mannan-binding lectin Innate immune system Complement System Proteins Cell Biology Cytotoxicity Tests Immunologic Virology biology.protein Mannose |
| Zdroj: | Biochemical and Biophysical Research Communications. 217:1128-1136 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.2886 |
| Popis: | The mechanism of lysis of influenza virus-infected BHK-21 cells by guinea pig serum (GPS) was investigated. Lysis was shown to involve activation of the classical complement pathway and was dependent on the presence of a mannose-binding lectin in GPS. FACS analysis demonstrated Ca(2+)-dependent binding of the lectin to influenza virus-infected, but not uninfected, cells. Cells infected with mutant strains of virus lacking a particular high-mannose oligosaccharide at the tip of the hemagglutinin molecule showed reduced binding of the lectin and were correspondingly less sensitive to lysis by GPS than cells infected with the parent viruses. The degree or pattern of glycosylation of influenza viruses thus influences susceptibility to this mechanism of viral clearance. By interfering with the infectious process, lectin-dependent complement-mediated lysis of infected cells may be an important component of innate immunity to influenza and other enveloped viruses. |
| Databáze: | OpenAIRE |
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