Flow cytometry analysis of pituitary adenomas
| Autor: | B. Oliver, M.E. Mato, X. Matías-Guiu, Frederic Bartumeus, Jordi Petriz, Oscar Fornas, D. Tugues, P. Tresserres, Susan M. Webb, M. Viader |
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| Předmět: |
Adenoma
Adult Male Pathology medicine.medical_specialty Endocrinology Diabetes and Metabolism Cell Dna index Biology Flow cytometry chemistry.chemical_compound Endocrinology Pituitary Hormones Anterior Proliferating Cell Nuclear Antigen medicine Animals Humans Pituitary Neoplasms Aged Ploidies medicine.diagnostic_test Cell growth Pituitary tumors DNA Neoplasm Middle Aged Flow Cytometry medicine.disease Immunohistochemistry Rats Proliferating cell nuclear antigen medicine.anatomical_structure chemistry biology.protein Female Ploidy Cell Division DNA |
| Zdroj: | Europe PubMed Central |
| Popis: | Using flow cytometry, DNA content and index, and/or proliferative capacity (measuring proliferating cell nuclear antigen PCNA) in operated pituitary tumors, control pituitaries obtained at necropsy, and experimental pituitary hyperplasia induced in rats were analyzed. Simultaneous measurement of cell ploidy and proliferation differentiated normal pituitary (diploid DNA index and negative PCNA) from pituitary hyperplasia (diploid DNA index with intensely positive PCNA, between 30 and 72% of cells). In the tumors 83% (19/ 23) were positive for PCNA (between 3 and 84%) and 73% (17/23) aneuploid; only 1 tumor was diploid and negative for PCNA. Conclusions: Differentiation between normal and abnormal (neoplastic or hyperplastic) pituitary is possible by flow cytometry, but in the adenomas no correlation with postoperative clinical outcome was observed. |
| Databáze: | OpenAIRE |
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