Restrictive water condition modifies the root exudates composition during peanut-PGPR interaction and conditions early events, reversing the negative effects on plant growth
| Autor: | Adriana Belén Cesari, Javier Hidalgo-Castellanos, Carmen Lluch Plá, Natalia Soledad Paulucci, Marta Susana Dardanelli, Miguel López-Gómez |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Exudate Early interaction events Arachis Physiology Microorganism purl.org/becyt/ford/1.7 [https] Plant Science Rhizobacteria Plant Roots 01 natural sciences Citric Acid purl.org/becyt/ford/1 [https] 03 medical and health sciences Arachis hypogaea Genetics medicine Bradyrhizobium Lactic Acid Symbiosis Microbial inoculant Legume Flavonoids Dehydration Indoleacetic Acids biology Chemistry Root exudate Otras Ciencias Naturales y Exactas Fatty Acids Tryptophan food and beverages Plant physiology biology.organism_classification Restrictive water condition Horticulture 030104 developmental biology Flavanones medicine.symptom CIENCIAS NATURALES Y EXACTAS Bacteria Oleic Acid 010606 plant biology & botany |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2019.08.015 |
| Popis: | Water deficit is one of the most serious environmental factors that affect the productivity of crops in the world. Arachis hypogaea is a legume with a high nutritional value and 70% is cultivated in semi-arid regions. This research aimed to study the effect of water deficit on peanut root exudates composition, analyzing the importance of exudates on peanut-PGPR interaction under restrictive water condition.Peanut seedlings were subjected to six treatments: 0 and 15 mM PEG, in combination with non-inoculated, Bradyrhizobium sp. and Bradyrhizobium-Azospirillum brasilense inoculated treatments. We analyzed the 7-day peanut root exudate in response to a water restrictive condition and the presence of bacterial inocula. Molecular analysis was performed by HPLC, UPLC and GC. Bacteria motility, chemotaxis, bacterial adhesion to peanut roots and peanut growth parameters were analyzed. Restrictive water condition modified the pattern of molecules exuded by roots, increasing the exudation of Naringenin, oleic FA, citric and lactic acid, and stimulation the release of terpenes of known antioxidant and antimicrobial activity. The presence of microorganisms modified the composition of root exudates. Water deficit affected the first events of peanut-PGPR interaction and the root exudates favored bacterial mobility, the chemotaxis and attachment of bacteria to peanut roots.Changes in the profile of molecules exuded by roots allowed A. hypogaea-Bradyrhizobium and A.hypogaea?Bradyrhizobium-Azospirillum interaction thus reversing the negative effects of restrictive water condition on peanut growth. These findings have a future potential application to improve plant-PGPR interactions under water deficit by formulating inoculants containing key molecules exuded during stress. Fil: Cesari, Adriana Belen. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Cordoba. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: Paulucci, Natalia Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Cordoba. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina Fil: López Gómez, Miguel. Universidad de Granada; España Fil: Hidalgo Catellano, Javier. Universidad de Granada; España Fil: Luch Plá, Carmen. Universidad de Granada; España Fil: Dardanelli, Marta Susana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Biotecnología Ambiental y Salud - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Cordoba. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina |
| Databáze: | OpenAIRE |
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