Sensitivity of Polyvoltine Thai Strains of Bombyx mori to a BmNPV Isolate From Mahasarakham
| Autor: | Siripuk Suraporn, Olle Terenius |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
AcademicSubjects/SCI01382 Veterinary medicine Severe disease Virulence 01 natural sciences Time to death Microbiology Virus 03 medical and health sciences polyvoltine Bombyx mori Animals 030304 developmental biology 0303 health sciences biology Inoculation Hexagonal crystal system Research fungi General Medicine biology.organism_classification Bombyx Thailand Nucleopolyhedroviruses virulence 010602 entomology BmNPV-MSU Mikrobiologi Insect Science Bombyx mori nucleopolyhedrovirus Host-Pathogen Interactions nucleopolyhedrovirus |
| Zdroj: | Journal of Insect Science |
| Popis: | Virus infection by the Bombyx mori nucleopolyhedrovirus (BmNPV) is the most severe disease in Thai sericultural practice of polyvoltine silkworms. Here, we characterized a newly isolated BmNPV isolated from the Mahasarakham province in Thailand (BmNPV-MSU). The purity and morphology of BmNPV-MSU were examined using light microscopy and scanning electron microscopy. The polyhedral inclusion bodies (PIBs) of BmNPV-MSU appeared in tetragonal, hexagonal, octagonal, and globular forms. The virions were both single and multiple embedded as observed by transmission electron microscopy. We also determined the virulence of BmNPV-MSU for six different Thai polyvoltine strains by LC50 and time to death after infection. The LC50 values of Nang Lai, NK04, and Sam Rong strains were 5.05–1.52 × 107 PIBs per ml and mortality peaked 7- to 8-d after inoculation. For Nang Noi, SP2, and RE05 strains the LC50 values were 7.91–1.82 × 106 PIBs/ml and mortality peaked 4–5 d after inoculation, thus having lower chance of survival to infection by BmNPV-MSU. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|