Surface modified silk fibroin nanoparticles for improved delivery of doxorubicin: Development, characterization, in-vitro studies
| Autor: | Avik Chakraborty, Sharmila Banerjee, Tanweer Haider, Ashok Chandak, Vikas Pandey, Vandana Soni |
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| Rok vydání: | 2020 |
| Předmět: |
Scanning electron microscope
Silk Fibroin Nanoparticle Polysorbates 02 engineering and technology Biochemistry Cell Line 03 medical and health sciences Mice Drug Delivery Systems Structural Biology Cell Line Tumor Animals Humans Surface charge Particle Size Molecular Biology 030304 developmental biology 0303 health sciences Chemistry Substrate (chemistry) General Medicine 021001 nanoscience & nanotechnology Rats Surface coating Drug Liberation RAW 264.7 Cells Transmission electron microscopy Doxorubicin Drug delivery Biophysics Cytokines Nanoparticles 0210 nano-technology Fibroins |
| Zdroj: | International journal of biological macromolecules. 164 |
| ISSN: | 1879-0003 |
| Popis: | Silk fibroin nanoparticles possess the hydrophobic nature which assists them to become a good substrate for reticulo-endothelial system (RES) and macrophageal uptake. Surface coating of these nanoparticles with hydrophilic stabilizers, like Tween-80 make them long circulating and facilitate their uptake by low density lipoprotein (LDL) receptors to cross blood brain barrier (BBB). Surface modified silk fibroin nanoparticles bearing anti-cancer agent doxorubicin (DOX) were fabricated by desolvation method and coated with Tween-80 as surface modifier. The prepared nanoparticles were characterized for various physicochemical parameters, like particle size, surface charge, surface morphology by scanning electron microscope (SEM) and transmission electron microscopy (TEM), and in vitro drug release along with in vitro cell cytotoxicity, flow cytometry and cellular uptake studies by flourocytometry on glioblastoma cell lines. Entrapment efficiency for the silk fibroin nanoparticles were found to be >85% for coated and uncoated nanoparticles. Nanoparticles with average diameter less than 150 nm having negative charge were found to show no toxicity of its own. The pro-inflammatory response of nanoparticles was observed by determining the cytokines level, such as TNF-α and IL-1β. Sustained drug release pattern from the nanoparticles with better cytotoxicty as compared to free drug was observed, signifying their potential ability to work as a drug delivery system. |
| Databáze: | OpenAIRE |
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