Ccq1-Raf2 interaction mediates CLRC recruitment to establish heterochromatin at telomeres
| Autor: | Yanjia Lu, Ming Lei, Jing Xue, Hong Sun, Zhenfang Wu, Shaohua Shi, Yuanze Zhou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Telomeric heterochromatin assembly
Ecology Heterochromatin Methyltransferase complex Health Toxicology and Mutagenesis Cell Cycle Proteins Plant Science Histone-Lysine N-Methyltransferase Biology Telomere Subtelomere Shelterin Chromatin Assembly and Disassembly Biochemistry Genetics and Molecular Biology (miscellaneous) Methylation Shelterin Complex Subtelomeric heterochromatin Cell biology Histone Schizosaccharomyces biology.protein Nucleosome Schizosaccharomyces pombe Proteins |
| Zdroj: | Life science alliance. 4(11) |
| ISSN: | 2575-1077 |
| Popis: | Telomeres, highly ordered DNA-protein complexes at eukaryotic linear chromosome ends, are specialized heterochromatin loci conserved among eukaryotes. In Schizosaccharomyces pombe, the shelterin complex is important for subtelomeric heterochromatin establishment. Despite shelterin has been demonstrated to mediate the recruitment of the Snf2/histone deacetylase–containing repressor complex (SHREC) and the Clr4 methyltransferase complex (CLRC) to telomeres, the mechanism involved in telomeric heterochromatin assembly remains elusive due to the multiple functions of the shelterin complex. Here, we found that CLRC plays a dominant role in heterochromatin establishment at telomeres. In addition, we identified a series of amino acids in the shelterin subunit Ccq1 that are important for the specific interaction between Ccq1 and the CLRC subunit Raf2. Finally, we demonstrated that the Ccq1–Raf2 interaction is essential for the recruitment of CLRC to telomeres, that contributes to histone H3 lysine 9 methylation, nucleosome stability and the shelterin-chromatin association, promoting a positive feedback mechanism for the nucleation and spreading of heterochromatin at subtelomeres. Together, our findings provide a mechanistic understanding of subtelomeric heterochromatin assembly by shelterin-dependent CLRC recruitment to chromosomal ends. |
| Databáze: | OpenAIRE |
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