NONO Is a Negative Regulator of SOX2 Promoter
| Autor: | Shanshan Liang, Jun-ichi Hamada, Tetsuro Hirose, Shigetsugu Hatakeyama, Hironori Yoshiyama, Hidehisa Takahashi, Yasuhiro Kuramitsu, Hisashi Iizasa, Ruoyu Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
SOX2 negative regulator transcription factor breast cancer cancer stem cell Regulator Breast Neoplasms Biology Biochemistry 03 medical and health sciences 0302 clinical medicine stomatognathic system Cancer stem cell Transcription (biology) Cell Line Tumor Biomarkers Tumor Genetics Humans Promoter Regions Genetic Molecular Biology Transcription factor Gene knockdown SOXB1 Transcription Factors fungi RNA-Binding Proteins Cell biology DNA-Binding Proteins Gene Expression Regulation Neoplastic Testis determining factor 030220 oncology & carcinogenesis embryonic structures Neoplastic Stem Cells Female sense organs biological phenomena cell phenomena and immunity Chromatin immunoprecipitation Research Article |
| Zdroj: | Cancer Genomics Proteomics |
| ISSN: | 1790-6245 1109-6535 |
| DOI: | 10.21873/cgp.20195 |
| Popis: | Background/aim Sex determining region Y (SRY)-box 2 (SOX2) is a transcription factor essential for the maintenance of proliferation and self-renewal of cancer stem cells and is associated with breast cancer initiation. Regulation of cancer stem cell plasticity by SOX2 requires both positive and negative SOX2 transcription factors, but the negative regulator is still largely unknown. Materials and methods SOX2 promoter-binding proteins were identified by liquid chromatography-mass spectrometry/mass spectrometry, luciferase assay, and chromatin immunoprecipitation. The effects of one such transcription factor on SOX2 expression was investigated by knockdown and overexpression experiments. Results Non-POU domain-containing octamer-binding protein (NONO) (also known as 54-kDa nuclear RNA-binding protein, P54NRB) was identified as a SOX2 promoter-binding protein and a negative regulator of SOX2 expression. Its activity was controlled by its coiled-coil domain and the C-terminal domain. Conclusion These results suggest that NONO acts as a key regulator of SOX2 transcription through the repression of SOX2 promoter activity in breast cancer cells. |
| Databáze: | OpenAIRE |
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