The role of bacteriophage T7 gene 2 protein in DNA replication

Autor:	R. North, Patrick Q. Mooney, Ian J. Molineux
Rok vydání:	1980
Předmět:	DNA Replication DNA Bacterial FOSL1 Biology Virus Replication Rifamycins Molecular biology GPS2 Cell biology Viral Proteins DDB1 EIF4EBP1 DNA Viral Escherichia coli Genetics AKT1S1 T-Phages Regulator gene HSPA9 TAF15
Zdroj:	Nucleic Acids Research. 8:3043-3054
ISSN:	1362-4962 0305-1048
Popis:	The in vivo function of the gene 2 protein of bacteriophage T7 has been examined. The gene 2 protein appears to modulate the activity of the gene 3 endonuclease in order to prevent the premature degradation of any newly-formed DNA concatemers. This modulation is not however a direct interacton between the two proteins. In single-burst experiments rifamycin can substitute for the gene 2 protein, allowing formation of fast-sedimenting replicative DNA intermediates and progeny phage production. This suggests that the sole function of the gene 2 protein is inhibition of the host RNA polymerase and that the latter enzyme directs or promotes the endonucleolytic action of the gene 3 protein.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7f4b55655bd04c6ec3ac7140e4b0fff https://doi.org/10.1093/nar/8.13.3043 Zobrazit plný text záznamu