Expression of Oct4 in human embryonic stem cells is dependent on nanotopographical configuration
| Autor: | Yen Peng Kong, Albert F. Yee, Christina H. Tu, Peter J. Donovan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Receptor crosstalk
Basic fibroblast growth factor Biomedical Engineering Biology Biochemistry Biomaterials Focal adhesion chemistry.chemical_compound Humans Nanotechnology Nanotopography Molecular Biology Cells Cultured Embryonic Stem Cells reproductive and urinary physiology Hexagonal crystal system General Medicine Embryonic stem cell Cell biology chemistry embryonic structures Microscopy Electron Scanning biological phenomena cell phenomena and immunity Octamer Transcription Factor-3 Focal adhesion formation Biotechnology Adult stem cell |
| Zdroj: | Acta Biomaterialia. 9:6369-6380 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2013.01.036 |
| Popis: | The fate of adult stem cells can be influenced by physical cues, including nanotopography. However, the response of human embryonic stem cells (hESCs) to dimensionally well-defined nanotopography is unknown. Using imprint lithography, we prepared well-defined nanotopography of hexagonal (HEX) and honeycomb (HNY) configurations with various spacings between the nanostructures. In serum-free hESC culture medium, basic fibroblast growth factor (bFGF) is required to maintain expression of Oct4, a pluripotent gene. Unexpectedly, hESCs cultured on nanotopography could maintain Oct4 expression without bFGF supplementation. With bFGF supplementation, the HEX nanotopography maintained Oct4 expression whereas the HNY configuration caused down-regulation of Oct4 expression. Thus, we observed that the lattice configurations of the nanotopography cause hESCs to respond to bFGF in different ways. This differential response to a biochemical cue by nanotopography was unforeseen, but its discovery could lead to novel differentiation pathways. Consistent with studies of other cells, we observed that nanotopography affects focal adhesion formation in hESCs. We posit that this can in turn affect cell–matrix tension, focal adhesion kinase signaling and integrin–growth factor receptor crosstalk, which eventually modulates Oct4 expression in hESCs. |
| Databáze: | OpenAIRE |
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