IL-23 directly enhances the proliferative and invasive activities of colorectal carcinoma
| Autor: | Kazuhiro Watanabe, Chikashi Shibata, Hitoshi Ogawa, Sho Haneda, Taiki Kajiwara, Toshihiro Komura, Hideyuki Suzuki, Shinobu Ohnuma, Tomohiko Sase, Iwao Sasaki, Hiroyuki Sasaki, Masahide Toshima, Koh Miura, Shunichi Kimura, Yasufumi Matsuda |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Oncogene business.industry Colorectal cancer medicine.medical_treatment Cancer Articles Mouse model of colorectal and intestinal cancer medicine.disease medicine.disease_cause Oncology Cancer immunotherapy Carcinoma medicine Autocrine signalling business Carcinogenesis |
| Zdroj: | Oncology Letters. 4:199-204 |
| ISSN: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2012.739 |
| Popis: | Interleukin-23 (IL-23) plays an essential role in the mucosal immune system. It has been suggested that IL-23 is able to induce carcinogenesis as well as inflammation and a recent study revealed that IL-23R is expressed in colorectal carcinoma cells. However, neither the differences in the IL-23R expression among the patients nor the concrete functions of IL-23 in colorectal carcinoma cells have been revealed. The aim of the present study was to examine the characteristics of IL-23R expression in colorectal carcinoma and the direct effects of IL-23 on colorectal cancer cells. We examined the IL-23R expression in human colorectal cancer tissue samples by immunohistochemistry. Cell proliferation and invasion assays under IL-23 stimulation were performed using cultured cells derived from colorectal cancer. ELISA and real-time PCR were used to evaluate the transforming growth factor (TGF)-β production due to IL-23 stimulation. All of the TNM stage IV patients were positive for IL-23R. IL-23R expression in the carcinoma tissue was also relatively high at the deepest point of invasion in certain cases. The proliferative and invasive activities and/or TGF-β production of DLD-1 cells increased by IL-23 stimulation, whereas no change was observed in the activities of MIP101 and KM12c cells. IL-23 directly enhanced the malignancy of the colon carcinoma cells. An autocrine mechanism via TGF-β production may underlie these effects. IL-23 is therefore a potential target for cancer immunotherapy. However, the homogeneity in IL-23R expression and the effects of IL-23 on colorectal carcinoma cells should be considered. |
| Databáze: | OpenAIRE |
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