Alterations of amino acid transmitter systems in spinal cords of chronic paraplegic dogs
| Autor: | J. T. Patrick, Peter V. Hall, E. Chernet, Scott Shapiro, William J. McBride |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
medicine.medical_specialty
Serotonin Serotonin uptake Synaptic Membranes Glutamic Acid Serotonergic Biochemistry Cellular and Molecular Neuroscience chemistry.chemical_compound Dogs Glutamates Internal medicine medicine Animals Spasticity Neurotransmitter gamma-Aminobutyric Acid chemistry.chemical_classification Paraplegia Neurotransmitter Agents business.industry Sodium Glutamate receptor Biological Transport Anatomy Spinal cord Amino acid Kinetics medicine.anatomical_structure Endocrinology chemistry Spinal Cord Female medicine.symptom business Synaptosomes |
| Zdroj: | Journal of neurochemistry. 42(6) |
| ISSN: | 0022-3042 |
| Popis: | The high-affinity uptake of [3H]serotonin, [3H]glutamate, and [3H]-γ-aminobutyric acid (3H]GABA) and the Na+-independent binding of [3H]glutamate and [3H]GABA were studied using spinal cord preparations obtained from normal mongrel dogs and from dogs made paraplegic by midthoracic spinal cord crush. Lumbosa-cral regions of the spinal cord were removed either before (1 week) or after (3 to 8 weeks) onset of spasticity. A myelin-free synaptosomal fraction was obtained by cen-trifugation and used for studying high-affinity uptake and for preparing synaptic plasma membranes for Na+-inde-pendent binding experiments. For the paraplegic groups, the uptake of 30 nM [3H]serotonin was 66 and 18% of control values after 1 and 3 weeks, respectively. Eadie-Hofstee analysis of [3H]serotonin uptake showed a 90% reduction in Vmax for the paraplegic group relative to control values, thereby indicating the expected loss of descending serotonergic pathways. The high-affinity uptakes of 1 μM [3H]glutamate and [3H]GABA were the same in both the control and nonspastic paraplegic groups after 1 week. However, after 3 weeks, the uptakes of [3H]glutamate and [3H]GABA were 60-70% higher for the spastic group than for the control animals. For both amino acids, Eadie-Hofstee plots revealed no difference in Km and higher Vmax for the spastic group relative to control values. After 1 and 3 weeks, the Na+ -independent binding of 5 nM [3H]glutamate was 40-85% higher and the binding of 10 nM [3H]GABA was 40-60% lower for the paraplegic groups relative to the values for the control animals. Scatchard analysis revealed significant changes in Bmax values for both amino acids. Overall, the data indicate an increase in segmental amino acid excitatory influence which occurred when signs of spasticity were evident. |
| Databáze: | OpenAIRE |
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