Antimicrobial susceptibility and genetic relatedness of respiratory tract pathogens in weaner pigs over a 12-month period
| Autor: | Michael Wendt, Jasmin Brauns, Doris Höltig, Lisa Niemann, Franziska Schäkel, Rolf Nathaus, Stefan Schwarz, Kristina Kadlec, Petra Müller, Kerstin Kipschull |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pasteurella multocida Streptococcus suis Tetracycline Swine 030106 microbiology Pasteurella Infections Erythromycin Microbial Sensitivity Tests Weaning Biology Bordetella bronchiseptica beta-Lactams Microbiology 03 medical and health sciences chemistry.chemical_compound Antibiotic resistance Anti-Infective Agents Drug Resistance Multiple Bacterial Streptococcal Infections medicine Animals Lincosamides Respiratory Tract Infections Bordetella Infections Swine Diseases General Veterinary Streptogramin B Bacteria Broth microdilution General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification 030104 developmental biology chemistry Macrolides medicine.drug |
| Zdroj: | Veterinary microbiology. 219 |
| ISSN: | 1873-2542 |
| Popis: | The collaboration project VASIB aims at reducing the antibiotic consumption in pig production by integrating information from consulting expertise in clinical inspection, hygiene, epidemiology, microbiology and pharmacology. In this VASIB subproject, we investigated the antimicrobial susceptibility and relatedness of porcine respiratory tract pathogens. Bordetella bronchiseptica (n = 47), Pasteurella multocida (n = 18) and Streptococcus suis (n = 58) were obtained from weaner pigs at two farms. Antimicrobial susceptibility testing was performed by broth microdilution according to CLSI standards. Resistance genes were detected via specific PCR assays. Macrorestriction analysis was conducted to determine the relatedness of the isolates and to identify clones. The B. bronchiseptica isolates showed indistinguishable (farm 1) or two closely related XbaI-patterns (farm 2). Different SmaI-PFGE patterns of P. multocida isolates were obtained at three different time points. In contrast, PFGE analysis of S. suis indicated more than one fragment pattern per pig and time point. Isolates exhibiting indistinguishable PFGE patterns were considered to represent the same clone. This study showed that only two closely related B. bronchiseptica clones were present in both farms, which had low MICs to all antimicrobials, except to β-lactams. Different P. multocida clones were present at the three time points. They showed overall low MIC values, with two clones being resistant and one intermediate to tetracycline. S. suis clones were resistant to tetracycline (n = 19) and/or erythromycin/clindamycin (n = 16). They harboured the tetracycline resistance genes tet(O), tet(M) or tet(L) and/or the macrolide/lincosamide/streptogramin B resistance gene erm(B). Five penicillin-resistant S. suis clones were also detected. |
| Databáze: | OpenAIRE |
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