Dienogest, a synthetic progestin, inhibits prostaglandin E2 production and aromatase expression by human endometrial epithelial cells in a spheroid culture system
| Autor: | Tatsuto Notsu, Satoru Kyo, Yutaka Shimizu, Shizuka Mita, Kiyoshi Mizuguchi, Takashi Takeuchi |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class medicine.medical_treatment Clinical Biochemistry Cell Culture Techniques Biology Biochemistry Dinoprostone Endometrium chemistry.chemical_compound Aromatase Endocrinology Spheroids Cellular Internal medicine Progesterone receptor medicine Humans Nandrolone Prostaglandin E2 Molecular Biology Cells Cultured Pharmacology Reverse Transcriptase Polymerase Chain Reaction Organic Chemistry Epithelial Cells Dienogest chemistry Estrogen Cell culture biology.protein Female lipids (amino acids peptides and proteins) Progestin Prostaglandin E medicine.drug |
| Zdroj: | Steroids. 76:60-67 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/j.steroids.2010.08.010 |
| Popis: | Prostaglandin E(2) (PGE(2)) is a major mediator in the pathophysiology, and pathogenesis of gynecological diseases associated with abnormal endometrial disease with proliferation and inflammation, such as endometriosis. In this study, we investigated the effect of dienogest, a selective progesterone receptor agonist, on PGE(2) production and the expression of aromatase, an estrogen synthase, in human immortalized endometrial epithelial cells. Compared with monolayer culture, the cells showed enhanced PGE(2) production and expression of the PGE(2) synthases cyclooxygenase-2 (COX-2), and microsomal prostaglandin E(2) synthase-1 (mPGES-1) in a spheroid culture system. Dienogest inhibited PGE(2) production and this effect was reversed by RU486, a progesterone receptor antagonist. Dienogest inhibited the PGE(2) synthases mRNA and protein expression, and the nuclear factor-κB activation. Moreover, the suppressive effect of dienogest on PGE(2) production was sustained 24h after the drug was withdrawn. Dienogest but not COX inhibitors inhibited aromatase expression. These results suggest that progesterone receptor activation reduces the gene expressions of COX-2, mPGES-1, and aromatase. Our findings suggest that the pharmacological mechanism of dienogest includes the direct inhibition of PGE(2) synthase and aromatase expression and may contribute to the therapeutic effect on the progression of endometriosis. |
| Databáze: | OpenAIRE |
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