miR-31, miR-155, and miR-221 Expression Profiles and Their Association With Graft Skin Tolerance in a Syngeneic vs Allogeneic Murine Skin Transplantation Model

Autor:	Edna Ayerim Mandujano-Tinoco, Francisco González-García, Rosa M Salgado, René Fernando Abarca-Buis, José Manuel Sanchez-Lopez, Pilar Hazel Carranza-Castro, Luis Padilla, Edgar Krötzsch
Rok vydání:	2022
Předmět:	Graft Rejection Mice Inbred BALB C Graft Survival Rehabilitation Hematopoietic Stem Cell Transplantation Skin Transplantation Mice Inbred C57BL Mice MicroRNAs Transforming Growth Factor beta3 Emergency Medicine Animals Transplantation Tolerance Surgery Burns
Zdroj:	Journal of Burn Care & Research. 43:1160-1169
ISSN:	1559-0488 1559-047X
DOI:	10.1093/jbcr/irac003
Popis:	Grafting is the preferred treatment for severe skin burns. Frequently, allogeneic tissue is the only transient option for wound coverage, but their use risks damage to surrounding tissues. MicroRNAs have been associated with acute rejection of different tissues/organs. In this study, we analyzed the expression of miR-31, miR-155, and miR-221 and associate it with graft tolerance or rejection using a murine full-thickness skin transplantation model. Recipient animals for the syngeneic and allogeneic groups were BALB/c and C57BL/6 mice, respectively; donor tissues were obtained from BALB/c mice. After 7 days posttransplantation (DPT), the recipient skin and grafts in the syngeneic group maintained most of their structural characteristics and transforming growth factor (TGF)-β1 and TGF-β3 expression. Allografts were rejected early (Banff grades II and IV at 3 and 7 DPT, respectively), showing damage to the skin architecture and alteration of TGF-β3 distribution. miRNAs skin expression changed in both mouse strains; miR-31 expression increased in the recipient skin of syngeneic grafts relative to that of allogeneic grafts at 3 and 7 DPT (P < .05 and P < .01, respectively); miR-221 expression increased in the same grafts at 7 DPT (P < .05). The only significant difference between donor tissues was observed for miR-155 expression at 7 DPT which was associated with necrotic tissue. Only miR-31 and miR-221 levels were increased in the blood of BALB/c mice that received syngeneic grafts after 7 DPT. Our data suggest that local and systemic miR-31 and miR-221 overexpression are associated with graft tolerance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b78647f0d02ba029c26bb11227b5a7c8 https://doi.org/10.1093/jbcr/irac003 Zobrazit plný text záznamu