Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
| Autor: | Ten Feizi, Lars P. Erwig, Sosthene Essono, Rodrigo Belmonte, Hillary Workman, Lisete M. Silva, Angelina S. Palma, Fiona M. Rudkin, Allan Jensen, Elizabeth M. Johnson, Ingrida Raziunaite, Donna M. MacCallum, Neil A. R. Gow |
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| Přispěvatelé: | UCIBIO - Applied Molecular Biosciences Unit, DQ - Departamento de Química |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemistry(all) medicine.drug_class Science General Physics and Astronomy Physics and Astronomy(all) Monoclonal antibody General Biochemistry Genetics and Molecular Biology Microbiology law.invention 03 medical and health sciences SDG 3 - Good Health and Well-being law medicine lcsh:Science Candida albicans B cell Multidisciplinary biology Biochemistry Genetics and Molecular Biology(all) Fungal genetics General Chemistry biology.organism_classification Disseminated Candidiasis 3. Good health 030104 developmental biology medicine.anatomical_structure Monoclonal biology.protein Recombinant DNA lcsh:Q Antibody |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-16 (2018) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Rudkin, F, Raziunaite, I, Workman, H, Essono, S, Belmonte, R, MacCallum, D M, Johnson, E M, Silva, L M, Palma, A S, Feizi, T, Jensen, A, Erwig, L P & Gow, N A R 2018, ' Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis ', Nature Communications, vol. 9, no. 1, 5288 (2018) . https://doi.org/10.1038/s41467-018-07738-1 |
| ISSN: | 2041-1723 |
| Popis: | FCT Investigator IF/00033/2012. Wellcome Trust (086827, 075470, 099215, 099197 and 101873) Wellcome Trust ISSF award (105625), MRC CiC (MC_PC_14114) and MRC Centre for Medical Mycology and University of Aberdeen for funding and a Wellcome Trust Strategic Award (097377). Wellcome Trust grant 099197MA . The high global burden of over one million annual lethal fungal infections reflects a lack of protective vaccines, late diagnosis and inadequate chemotherapy. Here, we have generated a unique set of fully human anti-Candida monoclonal antibodies (mAbs) with diagnostic and therapeutic potential by expressing recombinant antibodies from genes cloned from the B cells of patients suffering from candidiasis. Single class switched memory B cells isolated from donors serum-positive for anti-Candida IgG were differentiated in vitro and screened against recombinant Candida albicans Hyr1 cell wall protein and whole fungal cell wall preparations. Antibody genes from Candida-reactive B cell cultures were cloned and expressed in Expi293F human embryonic kidney cells to generate a panel of human recombinant anti-Candida mAbs that demonstrate morphology-specific, high avidity binding to the cell wall. The species-specific and pan-Candida mAbs generated through this technology display favourable properties for diagnostics, strong opsono-phagocytic activity of macrophages in vitro, and protection in a murine model of disseminated candidiasis. publishersversion published |
| Databáze: | OpenAIRE |
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