Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples

Autor:	Thais Martins Campos Araújo, Leonardo Alves Rusak, Cristina Barroso Hofer, Ernesto Hofer, Rodrigo de Castro Lisbôa Pereira, Deyse Christina Vallim, Isabelle Geoffroy Ribeiro Freitag, Larissa Alvarenga Batista Botelho
Rok vydání:	2020
Předmět:	Microbiology (medical) Enrichment broth Listeria medicine.disease_cause Microbiology Foodborne Diseases 03 medical and health sciences Feces Listeria monocytogenes medicine Humans Screening tool Food science Molecular Biology 030304 developmental biology 0303 health sciences Bacteriological Techniques biology 030306 microbiology Chemistry biology.organism_classification Culture Media Matrix-assisted laser desorption/ionization Food Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Food Microbiology Algorithms Brazil Contaminated food
Zdroj:	Journal of microbiological methods. 173
ISSN:	1872-8359
Popis:	We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b73a21523ceec493d1048884c47b3be2 https://pubmed.ncbi.nlm.nih.gov/32360382 Zobrazit plný text záznamu Full Text from ScienceDirect