Factor XIII Contrasts the Effects of Metalloproteinases in Human Dermal Fibroblast Cultured Cells
| Autor: | Monica De Mattei, Sergio Carandina, Angelo Caruso, Silvia Tognazzo, L. Fogato, Gian L. Scapoli, Alessia Ongaro, Massimiliano De Palma, Maria Luisa Serino, Paolo Zamboni, Donato Gemmati, Alberto Liboni |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Factor XIII
Metalloproteinases Dermal Fibroblast 030204 cardiovascular system & hematology NO 030218 nuclear medicine & medical imaging Dermal fibroblast 03 medical and health sciences 0302 clinical medicine Clostridium histolyticum medicine Humans Fibroblast Cells Cultured biology business.industry General Medicine Fibroblasts Middle Aged biology.organism_classification Cell counting Molecular biology Matrix Metalloproteinases Microbial Collagenase medicine.anatomical_structure Cell culture Microbial collagenase Immunology Collagenase Surgery Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Vascular and Endovascular Surgery. 38:431-438 |
| ISSN: | 1938-9116 1538-5744 |
| DOI: | 10.1177/153857440403800506 |
| Popis: | Matrix metalloproteinases (MMPs) are overexpressed in venous leg ulcers, determining a breakdown of the main extracellular matrix (ECM) components owing mainly to collagenase activities, and so playing a crucial role in ulcer pathogenesis. The authors studied the effects of coagulation factor XIII (FXIII), which cross-links collagen and other ECM components, in human fibroblast cultured cells in the presence and in the absence of matrix metalloproteinases from Clostridium histolyticum collagenase. Clostridium collagenase at concentrations of 2.0, 1.0, and 0.5 mg/mL was added to normal human dermal fibroblasts cultured in the presence of 0.0, 1.0, and 5.0 U/mL of FXIII concentrate (Fibrogammin P, Aventis Behring). Cell counting and metabolically active fibroblast evaluation in the cultures were monitored for 72 hours, by means of trypan-blue dye and MTT test, respectively. The MTT test showed that at the highest collagenase concentration (2.0 mg/mL), the cell number decreased more than 95% in 72 hours of treatment and no significant differences were observed regardless of the FXIII concentrations utilized. At lower collagenase concentration (1.0 mg/mL), in absence or in presence of FXIII (1.0 U/mL), the cell number decreased by about 80% in 72 hours. In contrast, in the presence of higher FXIII levels (5.0 U/mL), cells suffered globally significantly less collagenase effects (p=0.011) and the gain was appreciable at each time tested. Finally, at 0.5 mg/mL of collagenase concentration, in the absence of FXIII, the cell number decreased by about 60% in 72 hours, whereas in presence of FXIII 1.0 U/mL and 5.0 U/mL, cells decreased significantly less, by about 35% and 20%, respectively (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|