Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII
| Autor: | John B. McArthur, Stephanie Grond, Tilmann Weber, Thomas Schafhauser, Gavin J. Williams, Thomas Härtner, Wolfgang Wohlleben, Florian Zubeil, Sang Yup Lee, Ewa M. Musiol-Kroll, Irina Koryakina, Andreas Kulik |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pyridones Stereochemistry Biomedical Engineering Biochemistry Genetics and Molecular Biology (miscellaneous) Article 03 medical and health sciences chemistry.chemical_compound Polyketide Bacterial Proteins Biosynthesis Polyketide synthase Bacteria ATP synthase biology Chemistry General Medicine Anti-Bacterial Agents 030104 developmental biology Biochemistry Acyltransferases Polyketides Acyltransferase Click chemistry biology.protein Polyketide Synthases EF-Tu |
| Zdroj: | ACS Synthetic Biology. 6:421-427 |
| ISSN: | 2161-5063 |
| Popis: | During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses non-malonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by ‘click’ chemistry. |
| Databáze: | OpenAIRE |
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