The Relationship Between Environmental Factors and the Profile of Epstein-Barr Virus Antibodies in the Lytic and Latent Infection Periods in Healthy Populations from Endemic and Non-Endemic Nasopharyngeal Carcinoma Areas in China

Autor: Jiang Bo Zhang, Feng Hua Xu, Su Mei Cao, Qing Liu, Huan Lin Yu, Wen Qiong Xue, Qi Sheng Feng, Ya Fei Xu, Yong Qiao He, Yi Xin Zeng, Wei Hua Jia, Li Zhen Chen, Jianbing Mu
Rok vydání: 2018
Předmět:
0301 basic medicine
Adult
Male
China
Herpesvirus 4
Human
Asymptomatic males
lcsh:Medicine
Environment
medicine.disease_cause
Antibodies
Viral
General Biochemistry
Genetics and Molecular Biology
Virus
03 medical and health sciences
0302 clinical medicine
Antigen
Risk Factors
Cigarette smoking
hemic and lymphatic diseases
medicine
otorhinolaryngologic diseases
Humans
Lytic Phase
Antigens
Viral
lcsh:R5-920
Predictive marker
Nasopharyngeal Carcinoma
biology
lcsh:R
Carcinoma
Smoking
Nasopharyngeal Neoplasms
Anti-Epstein-Barr virus antibodies
General Medicine
Middle Aged
medicine.disease
Epstein–Barr virus
Immunoglobulin A
stomatognathic diseases
030104 developmental biology
Nasopharyngeal carcinoma
Lytic cycle
Epstein-Barr Virus Nuclear Antigens
030220 oncology & carcinogenesis
Immunology
biology.protein
Antibody
lcsh:Medicine (General)
Research Paper
Zdroj: EBioMedicine
EBioMedicine, Vol 30, Iss, Pp 184-191 (2018)
ISSN: 2352-3964
Popis: Our previous study found that smoking was associated with an elevated level of the antibody against VCA in the Epstein-Barr virus (EBV) lytic phase, which was an important predictive marker of the risk of nasopharyngeal carcinoma (NPC). It remained unknown whether environmental factors were associated with the levels of other EBV antibodies, such as Zta-IgA, EA-IgA, EBNA1-IgA, and LMP1-IgA, in the lytic and latent infection periods. We aimed to investigate the possible environmental inducers that could affect EBV antibody levels in two independent healthy male populations from endemic NPC areas in South China (N = 1498) and non-endemic NPC areas in North China (N = 1961). We performed ELISA and immunoenzymatic assays to test the levels of antibodies specific to the EBV antigens. The seropositive rates of antibodies against the antigens expressed in both the EBV latent and lytic infection periods, namely, LMP1-IgA, EBNA1-IgA, and Zta-IgA, in endemic areas (28.65%, 5.43% and 14.49%, respectively) were significantly higher than those in non-endemic areas (14.43%, 1.07% and 6.32%, respectively). Smoking was associated with higher seropositivity for EBNA1-IgA (OR = 1.47, 95% CI = 1.12–1.93) and Zta-IgA (OR = 1.28, 95% CI = 0.99–1.66), with dose-response effects, while not associated with the levels of LMP1-IgA. In conclusion, smoking was an important environmental factor, which associated with increased levels of EBNA1-IgA, and Zta-IgA.
Highlights • EBV antibodies against antigens were higher in healthy people in endemic areas than that in non-endemic areas of NPC. • Smoking was associated with higher seropositivity risk for EBNA1-IgA and Zta-IgA with dose-response relationships. • Smoking was not associated with seropositivity risk for LMP1-IgA. EBV, a class I oncovirus identified by WHO, is an important etiological factor of nasopharyngeal carcinoma (NPC). Numerous studies have demonstrated that higher levels of VCA-IgA, EBNA1-IgA, Zta-IgA against virus life cycle were good predict biomarkers for NPC risk. Our epidemiological study reported that several environmental factors, especially cigarette smoking, were associated the higher levels of Zta-IgA and EBNA1-IgA followed previously reported VCA-IgA against lytic period, the EBV reactivation marker; but not associated with LMP1-IgA, an antibody against a critical oncoprotein in latent period of EBV. It revealed that behavioral intervention, such as smoking cessation project, is very helpful in the primary prevention in endemic area.
Databáze: OpenAIRE
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