Immunogenicity of Attenuated Vesicular Stomatitis Virus Vectors Expressing HIV Type 1 Env and SIV Gag Proteins: Comparison of Intranasal and Intramuscular Vaccination Routes
| Autor: | Priscilla Piacente, Shakuntala Megati, Amjed Masood, Zimra R. Israel, John H. Eldridge, J. Erik Johnson, Dana L. Hasselschwert, Patricia A Reilly, John K. Rose, Kevin J Lopez, Preston A. Marx, David K. Clarke, David C. Montefiori, R. Michael Hendry, Stephen A. Udem, Mark G. Lewis, Eric M. Mishkin, Robert E Druilhet, Siew Yen Chong, Eva B. Schadeck, Paul W Barras, Nina F. Rose, Michael A. Egan |
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| Rok vydání: | 2004 |
| Předmět: |
T-Lymphocytes
Genetic Vectors Immunology Simian Acquired Immunodeficiency Syndrome Gene Products gag HIV Infections HIV Antibodies Antibodies Viral Vaccines Attenuated medicine.disease_cause Injections Intramuscular Vesicular stomatitis Indiana virus Virus Interferon-gamma Immune system Virology medicine Animals Humans Administration Intranasal AIDS Vaccines biology ELISPOT Immunogenicity Vaccination Gene Products env Simian immunodeficiency virus biology.organism_classification Macaca mulatta Infectious Diseases Vesicular stomatitis virus HIV-1 biology.protein Female Simian Immunodeficiency Virus Antibody |
| Zdroj: | AIDS Research and Human Retroviruses. 20:989-1004 |
| ISSN: | 1931-8405 0889-2229 |
| DOI: | 10.1089/aid.2004.20.989 |
| Popis: | An experimental AIDS vaccine based on attenuated, recombinant vesicular stomatitis virus (rVSV), when administered by a combination of parenteral and mucosal routes, has proven effective at preventing AIDS in a rhesus macaque model (Rose NF, et al.: Cell 2001;106:539-549). In an effort to determine the optimal route of vaccine administration we evaluated the ability of rVSV-based vaccine vectors expressing HIV-1 Env and SIV Gag proteins, when given either intramuscularly (i.m.) or intranasally (i.n.), to elicit antigen-specific cellular and humoral immune responses, and to protect from a subsequent vaginal challenge with simian-human immunodeficiency virus (SHIV89.6P). Our results demonstrate that macaques vaccinated by the i.n. route developed significantly higher antigen-specific cellular immune responses as determined by MHC class I tetramer staining, IFN-gamma ELISPOT, and cytotoxic T cell assays. However, systemic and mucosal humoral immune responses did not vary significantly with the route of vaccine administration. Given the importance of cell-mediated immune responses in slowing AIDS progression, intranasal delivery of a VSV-based AIDS vaccine may be an optimal as well as practical route for vaccination and should be considered in design of clinical trials. |
| Databáze: | OpenAIRE |
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