Skin integrity testing and monitoring ofin vitrotape stripping by capacitance-based sensor imaging
| Autor: | Perry Xiao, Silvia Haberfeld, Claudia Valenta, Julia C. Schwarz, Victoria Klang, Michael Wirth |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Materials science
Swine Analytical chemistry Dermoscopy Dermatology In Vitro Techniques Electric Capacitance Models Biological Capacitance Stripping (fiber) Spectroscopy Fourier Transform Infrared Microscopy Stratum corneum medicine Animals Humans Ear External Surgical Tape Penetration depth Barrier function Skin Transepidermal water loss integumentary system Proteins Water Water Loss Insensible Sweat Glands medicine.anatomical_structure Attenuated total reflection sense organs Densitometry Biomedical engineering |
| Zdroj: | Skin Research and Technology. 19:e259-e272 |
| ISSN: | 0909-752X |
| DOI: | 10.1111/j.1600-0846.2012.00637.x |
| Popis: | Background Despite the frequent use of porcine ear skin for tape-stripping experiments, the peculiarities of this skin type have not been characterised in detail yet. Thus, different techniques were employed to investigate the skin surface structure of porcine ear skin and the changes in barrier function during in vitro tape stripping. To this end, the potential of capacitance-based skin hydration imaging as a means of skin quality control was investigated for the first time. Methods The porcine ear model was characterised before and during tape stripping using transepidermal water loss (TEWL) measurements, capacitance-based sensor imaging, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and optical light microscopy. Results The capacitance-based sensor was found to deliver precise information about the quality of the employed skin sites before and during tape stripping. The removal of stratum corneum proteins was highly reproducible even for different porcine ear types. The mean greyscale values showed an excellent linear correlation to the corresponding TEWL values and the respective penetration depth. Optical light microscopy confirmed the presence of canyons on the surface of porcine ear skin. Conclusion The results suggest that the capacitance-based sensor is a suitable tool for skin integrity testing of porcine ear skin in vitro and for monitoring changes in skin barrier function. |
| Databáze: | OpenAIRE |
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