Mucosal Immunization with Live Recombinant Bovine Respiratory Syncytial Virus (BRSV) and Recombinant BRSV Lacking the Envelope Glycoprotein G Protects against Challenge with Wild-Type BRSV
| Autor: | Ulrike Schmidt, Ulf Polster, Laurel J. Gershwin, Jörg Beyer, Ursula J. Buchholz |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Genes
Viral Immunology Cattle Diseases Respiratory Syncytial Virus Bovine Respiratory Syncytial Virus Infections Antibodies Viral Virus Replication Microbiology law.invention Antigen Viral Envelope Proteins Immunity law Neutralization Tests Virology Vaccines and Antiviral Agents medicine Animals Antigens Viral Immunity Mucosal Lung Administration Intranasal Vaccines Synthetic biology Viral Vaccine Viral Vaccines medicine.anatomical_structure Immunization Viral replication Insect Science biology.protein Recombinant DNA Cattle Antibody Gene Deletion Respiratory tract |
| Popis: | Recombinant bovine respiratory syncytial virus (rBRSV) and an rBRSV deletion mutant lacking the G gene (rBRSVΔG) were characterized in calves with respect to replication competence, attenuation, and protective efficacy as live-attenuated BRSV vaccines. Both recombinant viruses were safe and induced protection against a BRSV challenge infection. rBRSV replicated efficiently in the upper respiratory tract. Intranasal immunization with rBRSVΔG led to infection but not to mucosal virus replication. Neutralizing antibodies were induced by rBRSV and rBRSVΔG. Thus, the BRSV attachment glycoprotein G seems to be dispensable in vaccinating calves against BRSV. |
| Databáze: | OpenAIRE |
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