Endoplasmic Reticulum Is Involved in Myocardial Injury in a Miniature Swine Model of Coronary Artery Stenosis Exposed to Acceleration-Associated Stress
| Autor: | Congchun Huang, Jin-Jin Sun, Meng Chai, Hai-tao Zhang, Yi Tian, Chao-zhong Liu, Huilan Luo, Xin-ya Yu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Swine Blotting Western Miniature swine lcsh:Medicine Apoptosis CHOP medicine.disease_cause Endoplasmic Reticulum chemistry.chemical_compound Internal medicine Malondialdehyde medicine Animals lcsh:Science Multidisciplinary TUNEL assay business.industry Superoxide Dismutase Endoplasmic reticulum Myocardium lcsh:R Coronary Stenosis medicine.disease Coronary arteries Stenosis Endocrinology medicine.anatomical_structure chemistry Swine Miniature lcsh:Q Female business Oxidative stress Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 7, p e0132654 (2015) |
| ISSN: | 1932-6203 |
| Popis: | This study aimed to investigate the effects of myocardial injury in a minimally-invasive miniature swine model with different levels of coronary artery stenosis (CAS) and exposed to maximal tolerated +Gz. Proximal left anterior descending branch was ligated in 20 swine. Five swine underwent a sham operation. A trapezoid acceleration curve was used for +Gz stress. Pathological changes of myocardial tissue were detected by H&E staining. Apoptotic cardiomyocytes were detected by TUNEL. GRP78 and CHOP were investigated by immunohistochemistry and western blot. CAS models were successful in 18 animals.Compared with the sham-operated group (+8.00±0.71 Gz), the maximal tolerated +Gz values of the moderate stenosis (+6.00±0.89 Gz, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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