Validated liquid chromatographic method and analysis of content of tilianin on several extracts obtained from Agastache mexicana and its correlation with vasorelaxant effect
| Autor: | Oswaldo Hernández-Abreu, Julio Cesar Rivera-Leyva, Samuel Estrada-Soto, Roberto Best-Brown, Rafael Villalobos-Molina, Liliana Durán-Gómez |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Agastache Vasodilator Agents Flavonoid Decoction High-performance liquid chromatography chemistry.chemical_compound Drug Discovery Maceration (wine) Animals Glycosides Rats Wistar Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Flavonoids Chromatography biology Chemistry Plant Extracts biology.organism_classification Rats Composition (visual arts) Agastache mexicana Lamiaceae Methanol |
| Zdroj: | Journal of ethnopharmacology. 138(2) |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance To optimize the obtention of tilianin, an antihypertensive flavonoid isolated from Agastache mexicana (Lamiaceae), a medicinal plant used in Mexico for the treatment of hypertension. Also, a validated HPLC method to quantify tilianin from different extracts, obtained by several extraction methods, was developed. Materials and methods The aerial parts of Agastache mexicana were dried at different temperatures (22, 40, 50, 90, 100 and 180 °C) and the dry material was extracted with methanol by maceration to compare the content of the active constituent tilianin in the samples. Furthermore, EtOH:H 2 O (7:3), infusion and decoction extracts were prepared from air-dried samples at room temperature to compare the content and composition of the different extraction methods. Moreover, an ex vivo vasorelaxant test on endothelium-intact aortic rat rings was conducted, in order to correlate the presence of tilianin with the activity of each extract. Results Higher concentration and amounts of tilianin were determined from chromatograms in the obtained methanolic extracts from plant material dried at 90, 50, 40 and 22 °C, followed by 100 °C; however, lower concentrations were observed in dried at 180 °C and EtOH:H 2 O (7:3). It is worth to notice that methanolic extracts with higher amount of tilianin were the most potent vasorelaxant extracts, even though these extracts were less potent than carbachol, a positive control used. Finally, decoction, infusion and EtOH:H 2 O (7:3) extracts did not show any vasorelaxant effect. Conclusion Results suggest that extracts with higher concentration of tilianin possess the best vasorelaxant activity, which allowed us to have a HPLC method for future quality control for this medicinal plant. |
| Databáze: | OpenAIRE |
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