Expression of mitochondrial genes MT-ND1, MT-ND6, MT-CYB, MT-COI, MT-ATP6, and 12S/MT-RNR1 in colorectal adenopolyps
| Autor: | LaShanale Wallace, Sharifeh Mehrabi, Methode Bacanamwo, Xuebiao Yao, Felix O. Aikhionbare |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Mitochondrial DNA Mitochondrion Biology Article Electron Transport Complex IV 03 medical and health sciences Gene expression Humans Gene Aged NADH Dehydrogenase General Medicine Cytochromes b Mitochondrial Proton-Translocating ATPases Molecular biology MT-CYB 030104 developmental biology RNA Ribosomal MT-ATP6 biology.protein Female MT-ND6 Colorectal Neoplasms Reactive Oxygen Species MT-ND1 |
| Zdroj: | Tumor Biology. 37:12465-12475 |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1007/s13277-016-5101-3 |
| Popis: | Despite improvements in treatment strategies, colorectal cancer (CRC) still has high mortality rates. Most CRCs develop from adenopolyps via the adenoma-carcinoma sequence. A mechanism for inhibition of this sequence in individuals with a high risk of developing CRC is urgently needed. Differential studies of mitochondrial (mt) gene expressions in the progressive stages of CRC with villous architecture are warranted to reveal early risk assessments and new targets for chemoprevention of the disease. In the present study, reverse transcription-quantitative PCR (RT-qPCR) was used to determine the relative amount of the transcripts of six mt genes [MT-RNR1, MT-ND1, MT-COI, MT-ATP6, MT-ND6, and MT-CYB (region 648–15887)] which are involved in the normal metabolism of mitochondria. A total of 42 pairs of tissue samples obtained from colorectal adenopolyps, adenocarcinomas, and their corresponding adjacent normal tissues were examined. Additionally, electron transport chain (ETC), complexes I (NADH: ubiquinone oxidoreductase) and III (CoQH2-cytochrome C reductase), and carbonyl protein group contents were analyzed. Results indicate that there were differential expressions of the six mt genes and elevated carbonyl protein contents among the colorectal adenopolyps compared to their paired adjacent normal tissues (p < 0.05). The levels of complexes I and III were higher in tumor tissues relative to adjacent normal tissues. Noticeably, the expression of MT-COI was overexpressed in late colorectal carcinomas among all studied transcripts. Our data suggest that increased expressions in certain mt genes and elevated levels of ROS may potentially play a critical role in the colorectal tumors evolving from adenopolyps to malignant lesions. |
| Databáze: | OpenAIRE |
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