The high performance liquid chromatography (HPLC) analysis of ultraviolet (UV) irradiated chlorophyll a and secondary plant compounds
| Autor: | Sarel F. Malan, Sandra van Dyk, Johanna C. Scheepers, Jan L. du Preez |
|---|---|
| Přispěvatelé: | 10065911 - Van Dyk, Sandra, 10199667 - Malan, Sarel Francois, 10060510 - Du Preez, Jan Lourens, 11311827 - Scheepers, Johanna Cornelia |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Chromatography Chlorophyll a medicine.disease_cause Applied Microbiology and Biotechnology High-performance liquid chromatography UV radiation Lipid peroxidation Chlorophyll a UV radiation activated charcoal HPLC secondary compounds in plant extracts chemistry.chemical_compound chemistry Activated charcoal secondary compounds in plant extracts Chlorophyll Genetics medicine Bioassay HPLC activated charcoal Agronomy and Crop Science Molecular Biology Reactive nitrogen species Ultraviolet Biotechnology |
| Zdroj: | African Journal of Biotechnology; Vol 10, No 74 (2011); 16976-16985 |
| ISSN: | 1684-5315 |
| Popis: | A large number of diseases such as cancer, cardiovascular disease, inflammatory disease, neurodegenerative disorders and ageing are consequences of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Deoxyribonucleic acid (DNA) damage, lipid peroxidation and protein damage are biomarkers for damage in the cell caused by ROS and RNS. Various plant extracts have been tested for anti-oxidant properties. Due to the chlorophyll in the extracts, false negative results from spectrophotometric tests are often observed. A pilot study was done to determine the stability of the secondary compounds with high performance liquid chromatography (HPLC) after the samples were bleached with ultraviolet (UV) radiation and treated with activated charcoal. The chlorophyll a was successfully degraded with UV radiation in all the samples leaving a bleached extract suitable for biological assays. http://www.academicjournals.org/AJB/PDF/pdf2011/23Nov/Scheepers%20et%20al.pdf http://dx.doi.org/10.5897/AJB11.1999 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|