Increased visfatin expression is associated with nuclear factor-kappa B and phosphatidylinositol 3-kinase in periodontal inflammation
| Autor: | Yildirim Karslioglu, Rahsan Ilikci, Ugur Musabak, Sait Yesillik, N. Işıl Saygun, Erkan Özcan |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Interleukin-1beta Nicotinamide phosphoribosyltransferase Inflammation Biology Real-Time Polymerase Chain Reaction Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Humans RNA Messenger Nicotinamide Phosphoribosyltransferase General Dentistry Periodontitis Tumor Necrosis Factor-alpha Kinase NF-kappa B 030206 dentistry medicine.disease Immunohistochemistry Chronic periodontitis 030104 developmental biology Endocrinology chemistry Chronic Periodontitis Cytokines Female Tumor necrosis factor alpha Phosphatidylinositol 3-Kinase medicine.symptom |
| Zdroj: | Clinical Oral Investigations. 21:1113-1121 |
| ISSN: | 1436-3771 1432-6981 |
| DOI: | 10.1007/s00784-016-1871-7 |
| Popis: | Visfatin is an adipocytokine that plays a role in regulating periodontal inflammation by as yet identified mechanisms. It has been suggested that visfatin mediates inflammation via activation of the nuclear factor-kappa B (NF-κB) and phosphatidylinositol 3-kinase (PI3k) signaling pathways which play a role in the inhibition of neutrophil apoptosis. The aim of this study was to investigate the expression of visfatin, NF-κB (NF-κB1 and NF-κB2), PI3k, tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β) in the tissue of healthy individuals and patients with periodontitis. Tissue biopsy samples were obtained from 21 patients with chronic periodontitis and from the gingiva of 19 healthy individuals undergoing crown lengthening. The mRNA expression levels of visfatin, NF-κB, PI3k, TNF-α, and IL-1β were evaluated by quantitative real-time PCR (qPCR). Also, visfatin protein expression was measured by immunohistochemistry. Both qPCR and immunohistochemistry results revealed that the visfatin expression was higher in the tissues with periodontitis than in healthy tissues (P |
| Databáze: | OpenAIRE |
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