Genetic Analysis of the CompletegagandenvGenes of HIV Type 1 Subtype C Primary Isolates from South Africa
| Autor: | Candice C. Sampson, Tania de Villiers, Susan Engelbrecht, Susan W. Barnett, Jan zur Megede, Estrelita Janse van Rensburg |
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| Rok vydání: | 2001 |
| Předmět: |
viruses
Molecular Sequence Data Immunology HIV Infections V3 loop Biology Genes env Genetic analysis Virus Disease Outbreaks South Africa Phylogenetics Virology Humans Amino Acid Sequence Cloning Molecular Gene Phylogeny Genetics Phylogenetic tree Nucleic acid sequence Sequence Analysis DNA Genes gag Infectious Diseases GenBank HIV-1 |
| Zdroj: | AIDS Research and Human Retroviruses. 17:1533-1547 |
| ISSN: | 1931-8405 0889-2229 |
| Popis: | South Africa has one of the fastest growing HIV-1 epidemics, with an estimated 4.7 million people infected. To better understand the genetic diversity of this epidemic and its potential impact on vaccine development, we have cloned and sequenced the complete gag and env genes of 13 primary virus isolates. Phylogenetic analysis of our sequences and 69 complete env genes from the Los Alamos and GenBank databases revealed multiple subclusters within subtype C. The V3 loop region was relatively conserved in all our strains when compared with other subtypes, but the region immediately downstream was highly variable. No intersubtype recombinant forms were observed when comparing the gag and env sequences. Characterization of the complete gag and env genes enabled us to select specific strains for further vaccine development. |
| Databáze: | OpenAIRE |
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