Recombinant-DNA-derived bovine growth hormone from Escherichia coli. 2. Biochemical, biophysical, immunological and biological comparison with the pituitary hormone
| Autor: | Por-Hsiung Lai, Richard R. Everett, Lawrence M. Souza, Janice M. Davis, Keith Langley, Thor F. Berg, L. F. Krabill, Jette Wypych |
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| Rok vydání: | 1987 |
| Předmět: |
Size-exclusion chromatography
Radioimmunoassay Biochemistry law.invention chemistry.chemical_compound law Animals Bovine somatotropin Sulfhydryl Compounds Amino Acids Sodium dodecyl sulfate Polyacrylamide gel electrophoresis Chromatography High Pressure Liquid chemistry.chemical_classification Isoelectric focusing Circular Dichroism Rats Inbred Strains Recombinant Proteins Rats Amino acid chemistry Growth Hormone Recombinant DNA Electrophoresis Polyacrylamide Gel Female Spectrophotometry Ultraviolet Isoelectric Focusing |
| Zdroj: | European Journal of Biochemistry. 163:323-330 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Bacterially synthesized, recombinant-DNA-derived bovine growth hormone (r-bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit-bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N-terminal sequence analysis, amino acid composition, isoelectric focusing, reverse-phase high-performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight-gain assay. In every respect the r-bGH appears to be virtually identical to pit-bGH. |
| Databáze: | OpenAIRE |
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