Decrypting the environmental sources of Mycobacterium canettii by high-throughput biochemical profiling

Autor: Fériel Bouzid, Djaltou Aboubaker Osman, Ahmed Loukil, Michel Drancourt
Přispěvatelé: Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre d’Etudes et de Recherche de Djibouti (CERD), FEDER PA 0000320 PRIMI
Rok vydání: 2019
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
Tuberculosis
Lymph Node
Biochemistry
Geographical Locations
Vegetables
Environmental Microbiology
Pathogen
2. Zero hunger
Multidisciplinary
biology
Eukaryota
Nontuberculous Mycobacteria
Plants
Africa
Eastern
Bacterial Typing Techniques
3. Good health
Actinobacteria
Phenotype
Mycobacterium tuberculosis complex
Mycobacterium ulcerans
Djibouti
Medicine
Research Article
Algae
Science
030106 microbiology
Mycobacterium Infections
Nontuberculous
Fruits
Microbiology
Mycobacterium tuberculosis
03 medical and health sciences
Medicinal plants
Microbial ecology
Animals
Humans
Tuberculosis
Nitrogen metabolism
Tuberculosis
Pulmonary
Nitrogen cycle
Disease Reservoirs
Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
High-Throughput Screening Assays
Metabolism
030104 developmental biology
People and Places
Africa
Mycobacterium
Zdroj: PLoS ONE
PLoS ONE, 2019, 14 (9), pp.e0222078. ⟨10.1371/journal.pone.0222078⟩
PLoS ONE, Vol 14, Iss 9, p e0222078 (2019)
PLoS ONE, Public Library of Science, 2019, 14 (9), pp.e0222078. ⟨10.1371/journal.pone.0222078⟩
ISSN: 1932-6203
Popis: International audience; Mycobacterium canettii is a smooth bacillus related to the Mycobacterium tuberculosis complex. It causes lymph nodes and pulmonary tuberculosis in patients living in countries of the Horn of Africa, including Djibouti. The environmental reservoirs of M. canettii are still unknown. We aimed to further decrypt these potential reservoirs by using an original approach of High-Throughput Carbon and Azote Substrate Profiling. The Biolog Phenotype profiling was performed on six clinical strains of M. canettii and one M. tuberculosis strain was used as a positive control. The experiments were duplicated and authenticated by negative controls. While M. tuberculosis metabolized 22/190 (11%) carbon substrates and 3/95 (3%) nitrogen substrates, 17/190 (8.9%) carbon substrates and three nitrogen substrates were metabolized by the six M. canettii strains forming the so-called corebiologome. A total at 16 carbon substrates and three nitrogen substrates were metabolized in common by M. tuberculosis and the six M. canettii strains. Moreover, at least one M. canettii strain metabo-lized 36/190 (19%) carbon substrates and 3/95 (3%) nitrogen substrates for a total of 39/285 (13%) substrates. Classifying these carbon and nitrogen substrates into ten potential environmental sources (plants, fruits and vegetables, bacteria, algae, fungi, nematodes, mol-lusks, mammals, insects and inanimate environment) significantly associated carbon and nitrogen substrates metabolized by at least one M. canettii strain with plants (p = 0.006). These results suggest that some plants endemic in the Horn of Africa may serve as ecological niches for M. canettii. Further ethnobotanical studies will indicate plant usages by local populations, then guiding field microbiological investigations in order to prove the definite environmental reservoirs of this opportunistic tuberculous pathogen.
Databáze: OpenAIRE
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