Comparative analysis of a Bordetella pertussis patient isolated strain and classical strains used in the pertussis vaccine
| Autor: | A. Pereira, Aparecida Santo Pietro Pereira, Sylvia Y. Bando, Denise V. Tambourgi, Carlos Alberto Moreira-Filho |
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| Rok vydání: | 2005 |
| Předmět: |
Electrophoresis
Bordetella pertussis Immunoblotting Microbiology Mice Vaccine strain medicine Animals Humans Whooping cough Pertussis Vaccine General Veterinary General Immunology and Microbiology biology Strain (chemistry) business.industry Public Health Environmental and Occupational Health biology.organism_classification medicine.disease Antibodies Bacterial Virology RAPD Infectious Diseases Vaccine Potency Molecular Medicine Pertussis vaccine Rabbits business Bacteria medicine.drug |
| Zdroj: | Vaccine. 23:4353-4358 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2005.03.033 |
| Popis: | A new Bordetella pertussis strain isolated from a whooping cough Brazilian patient was characterized under molecular and immunological aspects and compared with strains used in the production of whole-cell pertussis vaccine. The isolate, named 21A1, exhibited the bacteriological characteristics classically described for B. pertussis. RAPD and SDS-PAGE analysis showed similar DNA and proteic profiles between classical vaccinal strains and the new isolate. Comparative analysis about the efficacy of vaccines in protecting mice against the intracerebral challenge showed that the 21A1 vaccine was able to induce the highest mouse protection when compared with other B. pertussis vaccines. The results presented here indicate that the inclusion of selected new strain isolates in the composition of the B. pertussis vaccines can be an alternative to maintain or increase vaccine potency. |
| Databáze: | OpenAIRE |
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