Phytoplasma detection and quantification: Make it easy

Autor:	C. Lanzoni, Carlo Poggi Pollini, Massimo Turina, Stefano Minguzzi, Claudio Ratti
Přispěvatelé:	Ratti C., Minguzzi S., Lanzoni Chiara., Poggi Pollini C., Turina M.
Rok vydání:	2019
Předmět:	Microbiology (medical) biology DNA and RNA extraction Cell Biology Crude extract biology.organism_classification Virology Detection Phytoplasmas Infectious Diseases Phytoplasma Candidatus Phytoplasma mali TaqMan Parasitology Flavescence dorée Ecology Evolution Behavior and Systematics
Zdroj:	Phytopathogenic Mollicutes. 9:83
ISSN:	2249-4677 2249-4669
Popis:	When high numbers of analyses are required a rapid sample preparation method coupled to RT-qPCR allows to reduce the time and the costs for the phytoplasma detection in plant samples. A comparison between DNA and RNA contribution to qPCR detection revealed the higher input of the latter ensuring the maintenance of the sensitivity and specificity of the assay. The protocol has been validated for the detection and quantification of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’, ‘Ca. P. prunorum’, ‘Ca. P. solani’ and “flavescence dorée” phytoplasma by qPCR, RT-qPCR, ddPCR and ddRT-PCR techniques based on TaqMan chemistry.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5ee127780d06ff12f7834e92c4e5495 https://doi.org/10.5958/2249-4677.2019.00042.2 Zobrazit plný text záznamu