Monitoring Neutrophil-Expressed Cell Surface Esophageal Cancer Related Gene-4 after Severe Burn Injury

Autor: Andrew Baird, Todd W. Costantini, Raul Coimbra, Nicole E. Lopez, Brian P. Eliceiri, Jeanne G. Lee, Bruce Potenza, Alan Smith
Rok vydání: 2015
Předmět:
Male
Pathology
Burn injury
Neutrophils
medicine.medical_treatment
Pilot Projects
2.1 Biological and endogenous factors
Prospective Studies
Aetiology
Young adult
Prospective cohort study
Cancer
screening and diagnosis
education.field_of_study
medicine.diagnostic_test
Middle Aged
Esophageal cancer
Flow Cytometry
Neoplasm Proteins
Detection
Infectious Diseases
Cytokine
5.1 Pharmaceuticals
Development of treatments and therapeutic interventions
Burns
Adult
Microbiology (medical)
medicine.medical_specialty
Physical Injury - Accidents and Adverse Effects
Surgical Infection Society Articles
Clinical Sciences
Population
Flow cytometry
Young Adult
Rare Diseases
Clinical Research
medicine
Animals
Humans
education
business.industry
Tumor Suppressor Proteins
Membrane Proteins
medicine.disease
4.1 Discovery and preclinical testing of markers and technologies
Good Health and Well Being
Surgery
Digestive Diseases
business
Ex vivo
Zdroj: Surgical infections, vol 16, iss 6
ISSN: 1557-8674
1096-2964
Popis: BackgroundWe identified recently esophageal cancer related gene-4 (ECRG4) as a candidate cytokine that is expressed on the surface of quiescent polymorphonuclear leukocytes (PMNs) and shed in response to ex vivo treatment with lipopolysaccharide. To investigate the potential biologic relevance of changes in cell surface ECRG4 in human samples, we performed a pilot study to examine a population of burn patients in whom blood could be analyzed prospectively. We hypothesized that cutaneous burn injury would alter cell surface expression of ECRG4 on PMNs.MethodsPatients admitted with more than 20% total burn surface area (TBSA) (n = 10) had blood collected at the time of admission and weekly thereafter. For comparison, blood was obtained from a control group of healthy human volunteers (n = 4). We used flow cytometry to measure changes in ECRG4(+) PMNs from patients during recovery from injury. Esophageal cancer related gene-4 expression at each time point was compared with the patient's clinical status based on a Multiple Organ Dysfunction (MOD) score.ResultsEsophageal cancer related gene-4 was detected on the PMN surface of cells collected from healthy volunteers, however, within 48 h of admission after burn injury (n = 10 patients), the number of PMNs with cell surface ECRG4 was decreased. Esophageal cancer related gene-4 expression in PMNs was re-established over the course of patient recovery, unless complications occurred. In this case, the decrease in cell surface ECRG4(+) PMNs preceded the clinical diagnosis of infectious complications and was reflected by increased organ injury scores.ConclusionFrom a small sample set, we were able to determine that PMN cell surface ECRG4 expression was decreased after burn injury and returned to baseline during recovery from injury. Although larger studies are needed to define the role of ECRG4 in human PMNs further, this report is the first assessment of cell surface ECRG4 protein in a patient population to support analogous findings in animal studies.
Databáze: OpenAIRE
Externí odkaz: