Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Autor: Daria N. Poshina, Natallia V. Dubashynskaya, Yuri A. Anufrikov, Elena V Demyanova, Yaroslav A. Dubrovskii, Sergei V. Raik, Anna Y. Shasherina, Yury A. Skorik, Elena S. Shcherbakova
Rok vydání: 2021
Předmět:
QH301-705.5
02 engineering and technology
Polysaccharide
Article
Catalysis
Inorganic Chemistry
Chitosan
03 medical and health sciences
chemistry.chemical_compound
hyaluronic acid
ESKAPE pathogens
Hyaluronic acid
medicine
drug delivery system
Humans
Pseudomonas Infections
Surface charge
Biology (General)
Physical and Theoretical Chemistry
diethylaminoethyl chitosan
QD1-999
Molecular Biology
polyelectrolyte complexes
Spectroscopy
030304 developmental biology
chemistry.chemical_classification
Drug Carriers
0303 health sciences
antimicrobial activity
Colistin
polymyxin
Organic Chemistry
Cationic polymerization
General Medicine
021001 nanoscience & nanotechnology
Polyelectrolytes
Polyelectrolyte
In vitro
Anti-Bacterial Agents
Computer Science Applications
Chemistry
chemistry
Pseudomonas aeruginosa
0210 nano-technology
medicine.drug
Nuclear chemistry
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 8381, p 8381 (2021)
Volume 22
Issue 16
ISSN: 1422-0067
DOI: 10.3390/ijms22168381
Popis: Improving the therapeutic characteristics of antibiotics is an effective strategy for controlling the growth of multidrug-resistant Gram-negative microorganisms. The purpose of this study was to develop a colistin (CT) delivery system based on hyaluronic acid (HA) and the water-soluble cationic chitosan derivative, diethylaminoethyl chitosan (DEAECS). The CT delivery system was a polyelectrolyte complex (PEC) obtained by interpolymeric interactions between the HA polyanion and the DEAECS polycation, with simultaneous inclusion of positively charged CT molecules into the resulting complex. The developed PEC had a hydrodynamic diameter of 210–250 nm and a negative surface charge (ζ-potential = −19 mV)
the encapsulation and loading efficiencies were 100 and 16.7%, respectively. The developed CT delivery systems were characterized by modified release (30–40% and 85–90% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro experiments showed that the encapsulation of CT in polysaccharide carriers did not reduce its antimicrobial activity, as the minimum inhibitory concentrations against Pseudomonas aeruginosa of both encapsulated CT and pure CT were 1 μg/mL.
Databáze: OpenAIRE
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