Acute hypoxia-reoxygenation and vascular oxygen sensing in the chicken embryo
| Autor: | Carlos E. Blanco, Eduardo Villamor, Riazuddin Mohammed, Angel Cogolludo, Carlos Salinas, Dino A. Giussani |
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| Přispěvatelé: | Kindergeneeskunde, RS: GROW - R4 - Reproductive and Perinatal Medicine, MUMC+: MA Medische Staf Kindergeneeskunde (9), Villamor, Eduardo [0000-0003-1949-9123], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
chicken embryo
FETAL CARDIOVASCULAR DEFENSE Pulmonary Circulation Physiology Vasodilator Agents Vasodilation Chick Embryo 030204 cardiovascular system & hematology DISEASE Hypoxemia Signalling Pathways PULMONARY-HYPERTENSION 0302 clinical medicine Original Research Hyperoxia HIGH-ALTITUDE Arteries medicine.anatomical_structure Anesthesia Vasculature Sodium nitroprusside ARTERY REACTIVITY medicine.symptom medicine.drug Signal Transduction Cardiovascular Conditions Disorders and Treatments Nitroprusside medicine.medical_specialty Endothelium endothelium Biology Maternal Fetal and Neonatal Physiology MECHANISMS 03 medical and health sciences Physiology (medical) medicine.artery Internal medicine medicine Animals Lactic Acid EXPOSURE Oxygen signaling hypoxia Colforsin DUCTUS-ARTERIOSUS HYPOXEMIA Hypoxia (medical) Acetylcholine Oxygen Endocrinology Pulmonary artery hyperoxia DEVELOPMENTAL-CHANGES Endothelium Vascular 030217 neurology & neurosurgery Myograph |
| Zdroj: | Physiological Reports, 5(22):13501. Wiley Physiological Reports Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid |
| ISSN: | 2051-817X |
| DOI: | 10.14814/phy2.13501 |
| Popis: | Fetal/perinatal hypoxia is one of the most common causes of perinatal morbidity and mortality and is frequently accompannied by vascular dysfunction. However, the mechanisms involved have not been fully delineated. We hypothesized that exposure to acute hypoxia-reoxygenation induces alterations in vascular O-2 sensing/signaling as well as in endothelial function in the chicken embryo pulmonary artery (PA), mesenteric artery (MA), femoral artery (FA), and ductus arteriosus (DA). Noninternally pipped 19-day embryos were exposed to 10% O-2 for 30 min followed by reoxygenation with 21% O-2 or 80% O-2. Another group was constantly maintained at 21% O-2 or at 21% O-2 for 30 min and then exposed to 80% O-2. Following treatment, responses of isolated blood vessels to hypoxia as well as endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside and forskolin) relaxation were investigated in a wire myograph. Hypoxia increased venous blood lactate from 2.03 +/- 0.18 to 15.98 +/- 0.73 mmol/L (P |
| Databáze: | OpenAIRE |
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