Experimental Oral Transmission of Atypical Scrapie to Sheep
| Autor: | Christopher M. Vickery, Timm Konold, Stephen A. C. Hawkins, Linda A. Terry, Lisa C. Thurston, S. Jo Moore, Marion Simmons, John Spiropoulos, Melanie J. Chaplin, Leigh Thorne, Richard Lockey |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Pathology medicine.medical_specialty sheep Epidemiology lcsh:Medicine Mice Transgenic Spleen Scrapie Biology lcsh:Infectious and parasitic diseases Mice Oral administration scrapie agent medicine Animals Bioassay Distribution (pharmacology) lcsh:RC109-216 Prion protein Infectivity Transmission (medicine) oral administration infectivity Research lcsh:R Brain Virology alimentary system prions and related diseases Infectious Diseases medicine.anatomical_structure Animals Newborn atypical bioassay |
| Zdroj: | Emerging Infectious Diseases, Vol 17, Iss 5, Pp 848-854 (2011) Emerging Infectious Diseases |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | Such transmission results in peripheral tissue infectivity that is not detectable by current surveillance screening methods. To investigate the possibility of oral transmission of atypical scrapie in sheep and determine the distribution of infectivity in the animals’ peripheral tissues, we challenged neonatal lambs orally with atypical scrapie; they were then killed at 12 or 24 months. Screening test results were negative for disease-specific prion protein in all but 2 recipients; they had positive results for examination of brain, but negative for peripheral tissues. Infectivity of brain, distal ileum, and spleen from all animals was assessed in mouse bioassays; positive results were obtained from tissues that had negative results on screening. These findings demonstrate that atypical scrapie can be transmitted orally and indicate that it has the potential for natural transmission and iatrogenic spread through animal feed. Detection of infectivity in tissues negative by current surveillance methods indicates that diagnostic sensitivity is suboptimal for atypical scrapie, and potentially infectious material may be able to pass into the human food chain. |
| Databáze: | OpenAIRE |
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